试剂盒 > 酶联免疫试剂盒 > 人免疫球蛋白M(IgM)ELISA Kit
    • ELISA Kit
    • 人免疫球蛋白M(IgM)ELISA Kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人免疫球蛋白M(IgM)ELISA Kit

  • 英文名称:

    Human Immunoglobulin M,IgM ELISA Kit

  • 货号:
    CSB-E07976h
  • 别名:
    N/A
  • 规格:
    96T
  • 中文价格:
    3200
  • 种属:
    Human
  • 其他种属:
  • 待测物名称:
    Immunoglobulin M,IgM
  • 缩写:
    IgM
  • 检测范围:
    4.68 ng/ml-300 ng/ml
  • 灵敏度:
    1.17 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    300 2.753 2.769 2.761 2.641  
    150 2.404 2.428 2.416 2.296  
    75 1.905 1.911 1.908 1.788  
    37.5 1.281 1.293 1.287 1.167  
    18.75 0.726 0.729 0.728 0.608  
    9.37 0.465 0.472 0.469 0.349  
    4.68 0.288 0.294 0.291 0.171  
    0 0.121 0.119 0.120    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:5000 Average % 86  
    Range % 80-94  
    1:10000 Average % 97  
    Range % 91-105  
    1:20000 Average % 95  
    Range % 91-102  
    1:40000 Average % 94  
    Range % 86-101  
  • 回收率:
    The recovery of human IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-101  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 研究领域:
    Immunology
  • 引用文献:
    1. Effect of Lactococcus lactis and Enterococcus faecium on growth performance, digestive enzymes and immune response of grouper Epinephelus coioides , Y.-Z. SUN et al,Aquaculture Nutrition,2012, June; 18(3): 281-289
    2. Effects of heat-treatment on plasma rich in growth factors-derived autologous eye drop , Anitua E et al,Exp Eye Res,2013 Dec 15. pii: S0014-4835(13)00349-7.