试剂盒 > 酶联免疫试剂盒 > 人免疫球蛋白A(IgA)ELISA Kit
    • ELISA Kit
    • 人免疫球蛋白A(IgA)ELISA Kit
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人免疫球蛋白A(IgA)ELISA Kit

  • 英文名称:

    Human Immunoglobulin A,IgA ELISA Kit

  • 货号:
    CSB-E07985h
  • 别名:
    N/A
  • 规格:
    96T
  • 中文价格:
    3600
  • 种属:
    Human
  • 其他种属:
  • 待测物名称:
    Immunoglobulin A,IgA
  • 缩写:
    IgA
  • 蛋白功能1:
    Immunoglobulin
  • 检测范围:
    0.24 ng/ml-1000 ng/ml
  • 灵敏度:
    0.24 ng/ml
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 下载:
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.180 2.205 2.193 2.124  
    250 2.093 2.002 2.048 1.979  
    62.5 1.841 1.868 1.855 1.786  
    15.6 1.528 1.501 1.515 1.446  
    3.9 0.772 0.753 0.763 0.694  
    0.98 0.396 0.397 0.397 0.328  
    0.24 0.189 0.180 0.185 0.116  
    0 0.069 0.068 0.069    
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10000 Average % 89  
    Range % 85-94  
    1:20000 Average % 90  
    Range % 86-97  
    1:40000 Average % 96  
    Range % 90-103  
    1:80000 Average % 89  
    Range % 86-93  
  • 回收率:
    The recovery of human IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 89-94  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 研究领域:
    Immunology
  • 引用文献:
    1. ELISA TECHNIQUE FOR CLINICAL EVALUATION OF SERUM IMMUNOGLOBULIN-A IN IRAQI HYPERGLYCEMIA PATIENTS , Alhamd A K J.et al,WORLD JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES,Volume 4, Issue 12, 124-134