试剂盒 > 酶联免疫试剂盒 > 人游离甲状腺素(FT4)ELISA Kit
    • ELISA Kit
    • 人游离甲状腺素(FT4)ELISA Kit
    • The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.From:MeSH[provided by RefSeq]
    • Pubchem853
    • CAS no.51-48-9
    • Molecular FormulaC15H11I4NO4
    • Molecular Weight776.87002
  • 产品类型:
    ELISA Kit
  • 产品名称:

    人游离甲状腺素(FT4)ELISA Kit

  • 英文名称:

    Human Free Thyroxine,FT4 ELISA Kit

  • 货号:
    CSB-E05078h
  • 规格:
    96T
  • 中文价格:
    3600
  • 种属:
    Human
  • 待测物名称:
    Free Thyroxine,FT4
  • 缩写:
    FT4
  • 检测范围:
    4pmol/L-64pmol/L
  • 灵敏度:
    1.08 pmol/L
  • 反应时间:
    1-5h
  • 所需样本体积:
    50-100ul
  • 检测波长:
    450 nm
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  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/L OD1 OD2 Average  
    4 2.547 2.646 2.597  
    8 1.435 1.495 1.465  
    16 0.797 0.851 0.824  
    32 0.517 0.523 0.520  
    64 0.307 0.295 0.301  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 93
    Range % 85-99
    1:2 Average % 90
    Range % 87-100
    1:4 Average % 95
    Range % 88-98
    1:8 Average % 95
    Range % 86-98
  • 回收率:
    The recovery of human FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 96 91-98
    EDTA plasma (n=4) 96 93-102
  • 研究领域:
    Others