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SARS-CoV-2入侵人体潜在靶点汇总

浏览次数:1087      日期:2020年03月31日 15:52

自2019年底新型冠状病毒肺炎(COVID19)爆发以来,截至到2020年3月26日,全球感染人数已超过46万,死亡人数也已超过2万。这次疫情的爆发给全球的经济造成了不可估量的损失,同时也让人们的生活陷入了一片混乱。SARS-CoV-2是导致COVID19呼吸系统疾病的病原体。了解更多SARS-CoV-2,请戳>>目前既没有抗病毒的特效药,也没有预防的疫苗。而且不幸的是,科学界对SARS-CoV-2感染的分子细节知之甚少。

SARS-CoV-2入侵人体靶点研究现状

目前针对于SARS-CoV-2入侵人体的主流说法是,刺突蛋白(spike,S)是介导病毒入侵宿主细胞的关键蛋白。SARS-CoV-2是通过S蛋白找到宿主细胞受体与之结合,通过网格蛋白介导膜融合被内吞入细胞。而且宿主细胞与病毒表面蛋白配受体关系和亲和力决定了病毒的传染性和嗜细胞特性,而致病力与其侵入的宿主细胞类型及其功能相关。有关于SARS-CoV-2入侵人体的靶点,目前有最新研究支撑的是ACE2和CD147。

1、ACE2

目前SARS-CoV-2入侵人体门户靶点公认度最高的是血管紧张素转化酶2(ACE2)。2020年1月,科学家们通过晶体结构解析证实ACE2是SARS-CoV-2 S蛋白的受体,而且ACE2与新冠病毒的RBD(受体结合结构域)之间的亲和力,比其与SARS病毒的RBD的亲和力高10到20倍,这也是SARS-CoV-2超强感染性的原因。新冠病毒S蛋白以三聚体形态存在,每一个单体中约有1300多个氨基酸,其中300多个氨基酸构成了“受体结合结构域”(RBD),即S蛋白与ACE2相联结的地方 [1] [2] [3]了解更多ACE2,请戳 >>

但是ACE2通常仅表达于心、肾、血管内皮细胞,而COVID-19患者通常是呼吸道、消化道、肝、脾多器官受损,鼻咽拭子、唾液、尿液、粪便和血液均检出病毒核酸,这提示SARS-CoV-2在人体还有其他受体。

2、CD147

在严重急性呼吸器官综合征(SARS)冠状病毒入侵宿主细胞过程中,CD147分子可通过与CyPA的相互作用介导与其在HIV-1入侵中类似的作用机制。而CD147-拮抗肽9与HEK293细胞的结合率高,对SARS-CoV具有抑制作用。由于SARS-CoV-2与SARS-CoV具有相似的特征,陈志南研究团队对CD147在SARS-CoV-2侵袭宿主细胞中的可能作用进行了研究。并于2020年3月14日,在预印版平台BioRxiv上发表了题为“SARS-CoV-2 invades host cells via a novel route: CD147-spike protein”的文章。该实验结果表明,在宿主细胞上阻断CD147对新冠病毒具有抑制作用,而且CD147在促进该病毒对宿主细胞的侵袭中起重要作用。另外,表面等离子体共振分析则证实了CD147和S蛋白(SP)的相互作用[4]了解更多CD147,请戳 >>

3、其他靶点

尽管CD147可以在多个组织和器官中表达,比如恶性肿瘤、中枢神经、表皮细胞基底层、毛囊外根鞘细胞、精子头部、血液系统、消化系统及泌尿系统,可以参与肿瘤发生发展、组织重建、淋巴细胞应答、精子发生、病毒感染和发育早期神经系统功能调节。那这是不是意味着SARS-CoV-2入侵人体是不是就没有其他靶点了呢?

为了验证这一点,2020年3月22日,David E. Gordon等94位专家在生物学预印本期刊在线发表了一篇通过新冠病毒SARS-CoV-2蛋白与人蛋白相互作用筛选潜在治疗靶点的文章。文章作者来自加州大学旧金山分校(美国新冠病毒研究的重要中心之一)等多个单位,题目为“A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing”。

其实文章的思路很简单,但工作量极为庞大。研究人员在人类细胞中克隆、标记和表达了SARS-CoV-2的29个蛋白中的26个,并通过亲和纯化质谱法(AP-MS)鉴定了与每一种蛋白相互作用的人蛋白。共鉴定出332种高度可信的与病毒蛋白相互作用的人蛋白(PPIs),其中66个可以作为潜在靶点,这些蛋白可由69种现有FDA批准的药物、临床试验中的药物和/或临床前化合物靶向。宿主依赖因子介导病毒感染的鉴定可能为开发广泛有效的针对SARS-CoV-2和其他致命冠状病毒株的抗病毒治疗提供重要的分子靶点[5]

SARS-CoV-2蛋白及互作靶点一览表

SARS-CoV-2分离株的序列分析表明,该30kb基因组编码多达14个开放阅读框(Orfs)。5端的Orf1a / Orf1ab开放阅读框可编码多蛋白,这些蛋白会被蛋白水解处理成16个非结构蛋白(Nsp1-16),形成复制酶/转录酶复合物(RTC)。RTC由多种酶组成,包括Nsp3、Nsp5,Nsp7-Nsp8 复合物、Nsp12、Nsp13、Nsp14、Nsp15以及Nsp10 / Nsp16。在病毒基因组的3末端,从9个预测的亚基因组RNA中表达了多达13个Orf。这些包括四个结构蛋白:S、E、M和N,以及九种假定的辅助因子。在基因组成上,SARS-CoV-2基因组与SARS-CoV非常相似:每个都有一个Orf1ab编码16个预测的Nsps,每个都有四个典型的冠状病毒结构蛋白。但是,它们的3开放阅读框互补性不同:SARS-CoV-2具有Orf3b和Orf10,其Orf8是完整的,而SARS-CoV编码的是Orf8a和Orf8b [6] [7] [8]

下表中的26个SARS-CoV-2蛋白分别为:Nsp1、Nsp2、Nsp4、Nsp5、Nsp6、Nsp7、Nsp8、Nsp9、Nsp10、Nsp11、Nsp12、Nsp13、Nsp14、Nsp15、Spike (S)、E、N、M、Orf3a、Orf3b、Orf6、Orf7a、Orf8、Orf9b、Orf9c和Orf10,另外还有一个突变体Nsp C145A。

SARS-CoV Proteins Human Potential Interactome Targets
Nsp1 COLGALT1, PKP2, POLA1, POLA2, PRIM1, PRIM2
Nsp2 EIF4E2, FKBP15, GIGYF2, POR, RAP1GDS1, SLC27A2, WASHC4
Nsp4 ALG11, DNAJC11, IDE, NUP210, TIMM10, TIMM10B, TIMM29, TIMM9
Nsp5 HDAC2
Nsp5 C145A TRMT1, GPX1
Nsp6 ATP13A3, ATP5MG, ATP6AP1, SIGMAR1
Nsp7 ACSL3, AGPS, COMT, CYB5B, CYB5R3, DCAKD, DNAJC19, FAM162A, GNB1, GNG5, HS2ST1, LMAN2, MOGS, MTARC1, NAT14, NDUFAF2, PTGES2, QSOX2, RAB10, RAB14, RAB18, RAB1A, RAB2A, RAB5C, RAB7A, RAB8A, RALA, RHOA, SCARB1, SCCPDH, SELENOS, TOR1AIP1
Nsp8 AATF, ATE1, CCDC86, DDX10, EXOSC2, EXOSC3, EXOSC5, EXOSC8, HECTD1, LARP7, MEPCE, MPHOSPH10, MRPS2, MRPS25, MRPS27, MRPS5, NARS2, NGDN, NOL10, NSD , NSD2, SEPSECS, SRP19, SRP54, SRP72
Nsp9 DCAF7, EIF4H, FBLN5, FBN1, FBN2, GTF2F2, MAT2B, MIB1, NEK9, NUP214, NUP54, NUP58, EIF4H, NUP62, NUP88, SPART, ZNF503
Nsp10 AP2A2, AP2M1, ERGIC1, GFER, GRPEL1
Nsp11 TBCA
Nsp12 AKAP8, BCKDK, CRTC3, LARP4B, MYCBP2, PDZD11, PLEKHA5, PPIL3, PRRC2B, RBM41, RIPK1, SBNO1, SLU7, TCF12, TYSND1, UBAP2, UBAP2L, USP54, ZC3H7A, ZNF318
Nsp13 AKAP9, C1orf50, CDK5RAP2, CENPF, CEP112, CEP135, CEP250, CEP350, CEP43, CEP68, CIT, CLIP4, CNTRL, ERC1, FYCO1, GCC1, GCC2, GOLGA2, GOLGA3, GOLGB1, GORASP1, GRIPAP1, HOOK1, HSBP1, JAKMIP1, MIPOL1, NIN, NINL , PCNT, PDE4DIP, PRKACA, PRKAR2A, PRKAR2B, RDX, TBK1, TBKBP1, TLE1, TLE3, TLE5, USP13
Nsp14 GLA, IMPDH2, SIRT5
Nsp15 ARF6, NUTF2, RNF41
Spike GOLGA7, ZDHHC5
Orf3a ALG5, ARL6IP6, CLCC1, HMOX1, SUN2, TRIM59, VPS11, VPS39
Orf3b STOML2
E AP3B1, BRD2, BRD4, CWC27, SLC44A2, ZC3H18
N CSNK2A2, CSNK2B, DDX21, FAM98A, G3BP1, G3BP2, LARP1, MOV10, PABPC1, PABPC4, RBM28, RPL36, RRP9, SNIP1, UPF1
M AAR2, AASS, ACADM, AKAP8L, ANO6, ATP1B1, ATP6V1A, BZW2, COQ8B, ETFA, FAM8A1, FASTKD5, GGCX, INTS4, PITRM1, PMPCA, PMPCB, PSMD8, REEP5, REEP6, RTN4, SAAL1, SLC25A21, SLC30A7, SLC30A9, STOM, TARS2, TUBGCP2, TUBGCP3, YIF1A
Orf6 MTCH1, NUP98, RAE1
Orf7a HEATR3, MDN1
Orf8 ADAM9, ADAMTS1, CHPF, CHPF2, CISD3, COL6A1, DNMT1, EDEM3, EMC1, ERLEC1, ERO1B, ERP44, FBXL12, FKBP10, FKBP7, FOXRED2, GDF15, GGH, HS6ST2, HYOU1, IL17RA, INHBE, ITGB1, LOX, MFGE8, NEU1, NGLY1, NPC2, NPTX1, OS9, PCSK6, PLAT, PLD3, PLEKHF2, PLOD2, POFUT1, POGLUT2, POGLUT3, PUSL1, PVR, SDF2, SIL1, SMOC1, STC2, TM2D3, TOR1A, UGGT2
Orf9b BAG5, CHMP2A, CSDE1, DCTPP1, DPH5, MARK1, MARK2, MARK3, PTBP2, SLC9A3R1, TOMM70
Orf9c ABCC1, ACAD9, ALG8, BCS1L, DPY19L1, ECSIT, ERMP1, F2RL1, FAR2, GHITM, GPAA1, NDFIP2, NDUFAF1, NDUFB9, NLRX1, PIGO, PIGS, RETREG3, SCAP, SLC30A6, TAPT1, TMED5, TMEM39B, TMEM97, UBXN8, WFS1
Orf10 CUL2, ELOB, ELOC, MAP7D1, PPT1, RBX1, THTPA, TIMM8B, ZYG11B

332新冠入侵人体潜在靶点产品目录

  • 蛋白产品
靶点 货号 种属 来源 表达区域 标签
AATF  CSB-RP052644h Human E.coli 6-294aa N-terminal GST-tagged
ABCC1 CSB-EP001056HU Human E.coli 1248-1531aa N-terminal 6xHis-tagged
ACADM CSB-EP001126HU Human E.coli 26-421aa N-terminal 6xHis-tagged
AP2M1 CSB-EP001872HU Human E.coli 1-435aa N-terminal 6xHis-SUMO-tagged
ARF6 CSB-EP001996HU Human E.coli 1-175aa N-terminal 6xHis-SUMO-tagged
BAG5 CSB-EP890743HU Human E.coli 1-447aa N-terminal 6xHis-SUMO-tagged
BZW2 CSB-EP896544HU Human E.coli 1-419aa N-terminal GST-tagged
CENPF CSB-BP005210HU Human Baculovirus 1759-2093aa N-terminal MBP-tagged and C-terminal 6xHis-tagged
CHMP2A CSB-RP045244h Human E.coli 1-222aa N-terminal GST-tagged
COMT CSB-EP005779HU Human E.coli 52-271aa N-terminal 6xHis-tagged
CSNK2B CSB-RP016554h Human E.coli 2-215aa N-terminal GST-tagged
DCAF7 CSB-EP026056HU Human E.coli 1-342aa N-terminal 6xHis-SUMO-tagged
DNAJC19 CSB-EP853400HU Human E.coli 1-116aa N-terminal GST-tagged
ELOB CSB-EP620878HU Human E.coli 1-118aa N-terminal GST-tagged
ELOC CSB-EP023281HU Human E.coli 1-112aa N-terminal GST-tagged
ERP44 CSB-EP861125HU Human E.coli 30-406aa N-terminal 6xHis-SUMO-tagged
ETFA  CSB-EP007844HU Human E.coli 1-333aa N-terminal GST-tagged
GCC1 CSB-EP846598HU Human E.coli 4-775aa N-terminal 6xHis-SUMO-tagged
GDF15 CSB-EP859530HU Human E.coli 198-308aa N-terminal 6xHis-tagged
GIGYF2 CSB-YP751088HU Human Yeast 510-1030aa N-terminal 6xHis-tagged
GLA CSB-EP009474HU Human E.coli 32-429aa N-terminal 6xHis-tagged
GPX1 CSB-EP009866HU Human E.coli 1-203aa N-terminal 6xHis-tagged
GRPEL1 CSB-EP875707HU Human E.coli 1-217aa N-terminal GST-tagged
GTF2F2 CSB-EP010005HU Human E.coli 2-249aa N-terminal 6xHis-SUMO-tagged
HMOX1 CSB-EP010583HU Human E.coli 3-288aa N-terminal 6xHis-tagged
HSBP1 CSB-RP004544h Human E.coli 1-75aa N-terminal GST-tagged
IMPDH2 CSB-RP011954h Human E.coli 5-514aa N-terminal GST-tagged
LOX CSB-RP169094h Human E.coli 174-417aa N-terminal 6xHis-tagged
MFGE8 CSB-EP013752HU Human E.coli 24-387aa N-terminal 6xHis-SUMO-tagged
MIB1 CSB-RP132874h Human E.coli 5-332aa N-terminal 6xHis-tagged
NUP210 CSB-EP016195HU(C) Human E.coli 1529-1808aa N-terminal 6xHis-tagged
NUTF2 CSB-EP016214HU Human E.coli 1-127aa N-terminal GST-tagged
PABPC1 CSB-EP017352HU Human E.coli 1-370aa N-terminal 6xHis-SUMO-tagged
PABPC4 CSB-EP615676HUe1 Human E.coli 1-644aa Tag-Free
PDE4DIP CSB-EP716571HU Human E.coli 1-310aa N-terminal GST-tagged
PDZD11 CSB-EP707840HU Human E.coli 1-140aa N-terminal 6xHis-SUMO-tagged
PLAT CSB-EP018120HU Human E.coli 36-556aa N-terminal 6xHis-SUMO-tagged
PLEKHF2 CSB-EP884492HU Human E.coli 1-249aa N-terminal GST-tagged
POR CSB-EP018378HU Human E.coli 2-671aa N-terminal GST-tagged
PRIM1 CSB-EP018680HU Human E.coli 1-420aa N-terminal 6xHis-SUMO-tagged
PRKACA CSB-EP018688HU Human E.coli 2-351aa N-terminal 6xHis-tagged
PVR CSB-EP019093HU Human E.coli 21-343aa N-terminal 6xHis-SUMO-tagged
RAB10 CSB-EP019152HU Human E.coli 1-196aa N-terminal 6xHis-tagged
RAB18 CSB-EP878835HU Human E.coli 1-206aa N-terminal GST-tagged
RAB1A CSB-RP003344h Human E.coli 2-205aa N-terminal GST-tagged
RAB2A CSB-EP019180HU Human E.coli 2-212aa N-terminal 6xHis-SUMO-tagged
RAB5C CSB-EP019215HU Human E.coli 1-216aa N-terminal 6xHis-SUMO-tagged
RAB8A CSB-EP019222HU(F1) Human E.coli 3-193aa N-terminal 6xHis-SUMO-tagged
RAE1 CSB-EP019276HU Human E.coli 1-368aa N-terminal GST-tagged
RBX1 CSB-EP019496HU Human E.coli 1-108aa N-terminal GST-tagged
RHOA CSB-RP008754h Human E.coli 1-191aa N-terminal GST-tagged
RIPK1 CSB-EP618785HU2 Human E.coli 1-375aa N-terminal 10xHis-tagged and C-terminal Myc-tagged
RNF41 CSB-EP880976HU Human E.coli 1-317aa N-terminal GST-tagged
SCARB1 CSB-EP845139HU Human E.coli 33-443aa N-terminal 6xHis-SUMO-tagged
SDF2 CSB-EP020900HU Human E.coli 19-211aa N-terminal 6xHis-tagged
SEPSECS CSB-RP143894h Human E.coli 5-451aa N-terminal 6xHis-tagged
SLC27A2 CSB-EP021534HU Human E.coli 283-620aa N-terminal 6xHis-SUMO-tagged
SRP19 CSB-EP022674HU Human E.coli 2-144aa N-terminal 6xHis-SUMO-tagged
SRP54  CSB-EP022675HU Human E.coli 1-504aa N-terminal 6xHis-B2M-tagged
SRP72 CSB-EP022677HU Human E.coli 2-671aa N-terminal GST-tagged
STOM CSB-EP022862HU Human E.coli 1-288aa N-terminal 6xHis-SUMO-tagged
TARS2 CSB-EP023132HU Human E.coli 369-718aa N-terminal 6xHis-SUMO-tagged
TIMM10B CSB-EP896532HU Human E.coli 1-103aa N-terminal 6xHis-SUMO-tagged
TLE5 CSB-EP001405HU Human E.coli 1-264aa N-terminal GST-tagged
  • 抗体产品
产品名称 产品货号 种属 种属反应性 应用
AAR2 Antibody CSB-PA896704LA01HU Human Human ELISA, IHC
AASS Antibody CSB-PA883373LA01HU Human Human ELISA, WB, IHC
AATF Antibody CSB-PA05264A0Rb Human Human ELISA, IHC
ACAD9 Antibody CSB-PA887999ESR1HU Human Human ELISA, WB, IHC
ACADM Antibody CSB-PA001126LA01HU Human Human ELISA, IHC
ADAM9 Antibody CSB-PA618774ESR1HU Human Human ELISA, WB, IHC
ADCK4 Antibody CSB-PA839305LA01HU Human Human ELISA, WB, IHC, IF
AES Antibody CSB-PA001405ESR2HU Human Human ELISA, IHC
AGPS Antibody CSB-PA001457LA01HU Human Human ELISA, WB, IHC, IF
AKAP8L Antibody CSB-PA892152ESR1HU Human Human ELISA, IHC
AKAP9 Antibody CSB-PA857882LA01HU Human Human ELISA, WB, IHC, IF
ALG11 Antibody CSB-PA647192LA01HU Human Human ELISA, IHC, IF
ALG5 Antibody CSB-PA896924LA01HU Human Human ELISA, IHC
ANO6 Antibody CSB-PA679761LA01HU Human Human ELISA, IF
AP2A2 Antibody CSB-PA001870DSR2HU Human Human, Mouse ELISA, WB, IHC
AP2M1 Antibody CSB-PA00625A0Rb Human Human ELISA, WB, IHC
AP3B1 Antibody CSB-PA001874ESR1HU Human Human ELISA, IHC
ARF6 Antibody CSB-PA001996LA01HU Human Human ELISA, WB, IHC
ATE1 Antibody CSB-PA002268LA01HU Human Human ELISA, IHC, IF
ATP1B1 Antibody CSB-PA002326LA01HU Human Human, Rat, Mouse ELISA, WB, IHC, IF
ATP5L Antibody CSB-PA002374LA01HU Human Human ELISA, IHC
BAG5 Antibody CSB-PA890743HA01HU Human Human, Mouse ELISA, WB, IHC
BCKDK Antibody CSB-PA002607ESR2HU Human Human ELISA, WB, IHC
BCS1L Antibody CSB-PA897463ESR1HU Human Human ELISA, WB, IHC
BRD4 Antibody CSB-PA002802LA01HU Human Human ELISA, IF
CDK5RAP2 Antibody CSB-PA847226LA01HU Human Human ELISA, IHC, IF
CEP135 Antibody CSB-PA721063LA01HU Human Human ELISA, IF
CEP250 Antibody CSB-PA871581LA01HU Human Human ELISA, IF
CEP68 Antibody CSB-PA765741LA01HU Human Human ELISA
CHMP2A Antibody CSB-PA04524A0Rb Human Human ELISA, WB, IHC
CLCC1 Antibody CSB-PA857024LA01HU Human Human ELISA, WB, IHC, IF, IP
CRTC3 Antibody CSB-PA747623LA01HU Human Human ELISA, IHC
CSDE1 Antibody CSB-PA006040LA01HU Human Human ELISA, IHC, IF
CSNK2A2 Antibody CSB-PA526319HA01HU Human Human ELISA, IHC, IF
CSNK2B Antibody CSB-PA01655A0Rb Human Human ELISA, IHC
CUL2 Antibody CSB-PA623822LA01HU Human Human ELISA, WB, IHC
CYB5B Antibody CSB-PA006310LA01HU Human Human, Mouse ELISA, WB, IHC, IF
CYB5R3 Antibody CSB-PA02655A0Rb Human Human ELISA, WB, IHC, IF
DCAF7 Antibody CSB-PA026056ESR2HU Human Human ELISA, IHC
DCAKD Antibody CSB-PA855481LA01HU Human Human ELISA
DNAJC19 Antibody CSB-PA853400ESR1HU Human Human, Mouse ELISA, WB, IHC, IP
DNMT1 Antibody CSB-PA007082HA01HU Human Human ELISA, IHC, IF
DPH5 Antibody CSB-PA862001LA01HU Human Human ELISA, IHC, IF
DPY19L1 Antibody CSB-PA23079A0Rb Human Human ELISA, IF
ECSIT Antibody CSB-PA859414ESR2HU Human Human ELISA, WB, IHC
EIF4E2 Antibody CSB-PA007558ESR1HU Human Human ELISA, IHC
ELOB Antibody CSB-PA620878ESR1HU Human Human ELISA, WB, IHC
ERGIC1 Antibody CSB-PA842630LA01HU Human Human ELISA, WB, IHC, IF
ERMP1 Antibody CSB-PA007794ESR1HU Human Human ELISA, IHC
ERO1B Antibody CSB-PA774834LA01HU Human Human, Mouse ELISA, WB, IHC
ETFA Antibody CSB-PA007844ESR1HU Human Human ELISA, WB, IHC
EXOSC3 Antibody CSB-PA889083LA01HU Human Human ELISA, IHC
EXOSC5 Antibody CSB-PA878857LA01HU Human Human ELISA, WB, IHC
EXOSC8 Antibody CSB-PA853387LA01HU Human Human, Mouse ELISA, WB
F2RL1 Antibody CSB-PA007925LA01HU Human Human ELISA, IHC, IF
FAR2 Antibody CSB-PA822239LA01HU Human Human ELISA, IHC, IF
FASTKD5 Antibody CSB-PA759173LA01HU Human Human ELISA, IHC
FBN1 Antibody CSB-PA008456LA01HU Human Human ELISA, IHC
FBN2 Antibody CSB-PA008457LA01HU Human Human ELISA, IF
FKBP10 Antibody CSB-PA822180LA01HU Human Human ELISA, IHC
FOXRED2 Antibody CSB-PA816892HA01HU Human Human ELISA, IHC
FYCO1 Antibody CSB-PA866262LA01HU Human Human, Mouse ELISA, WB, IHC
G3BP1 Antibody CSB-PA613389ESR1HU Human Human ELISA, WB, IHC
GCC1 Antibody CSB-PA846598LA01HU Human Human ELISA, WB, IHC
GDF15 Antibody CSB-PA10029A0Rb Human Human ELISA, WB, IHC
GFER Antibody CSB-PA009370ESR2HU Human Human, Mouse ELISA, WB, IHC
GGCX Antibody CSB-PA009388LA01HU Human Human ELISA, IHC
GGH Antibody CSB-PA009389LA01HU Human Human ELISA, WB
GHITM Antibody CSB-PA872487LA01HU Human Human, Rat ELISA, WB, IHC, IF
GLA Antibody CSB-PA009474LA01HU Human Human ELISA, IHC
GNB1 Antibody CSB-PA009602LA01HU Human Human, Mouse, Rat ELISA, WB, IF
GNG5 Antibody CSB-PA009617LA01HU Human Human ELISA, IHC, IF
GOLGA2 Antibody CSB-PA600856ESR1HU Human Human ELISA, IHC
GOLGA3 Antibody CSB-PA600096LA01HU Human Human ELISA, IHC, IF
GOLGB1 Antibody CSB-PA622788LA01HU Human Human ELISA, IHC, IF
GORASP1 Antibody CSB-PA861116ESR2HU Human Human ELISA, IHC
GPX1 Antibody CSB-RA009866A0HU Human Human ELISA
GRPEL1 Antibody CSB-PA875707ESR1HU Human Human ELISA, WB, IHC
GTF2F2 Antibody CSB-PA010005LA01HU Human Human, Mouse ELISA, WB
HMOX1 Antibody CSB-PA010583LA01HU Human Human ELISA, IHC, IF
HOOK1 Antibody CSB-PA891526LA01HU Human Human ELISA, WB, IHC
HS2ST1 Antibody CSB-PA010746LA01HU Human Human, Rat ELISA, WB, IHC
HSBP1 Antibody CSB-PA00454A0Rb Human Human ELISA, IHC
HYOU1 Antibody CSB-PA896895ESR1HU Human Human ELISA, IHC
IDE Antibody CSB-PA010988LA01HU Human Human ELISA, IHC, IF
IL17RA Antibody CSB-PA12929A0Rb Human Human, Mouse ELISA, WB, IF
IMPDH2 Antibody CSB-PA011701LA01HU Human Human, Mouse ELISA, WB, IHC, IF
ITGB1 Antibody CSB-RA011880A0HU Human Human ELISA, WB, IHC, IF, FC
LARP7 Antibody CSB-PA672326LA01HU Human Human ELISA, IHC
LOX Antibody CSB-PA16909A0Rb Human Human ELISA, WB, IHC
MARK1 Antibody CSB-PA873719DSR1HU Human Human, Rat ELISA, WB, IHC
MARK2 Antibody CSB-PA762568LA01HU Human Human ELISA, WB, IHC
MARK3 Antibody CSB-PA013498LA01HU Human Human ELISA, IHC, IF
MAT2B Antibody CSB-PA882157LA01HU Human Human, Rat ELISA, WB, IF
MFGE8 Antibody CSB-PA07974A0Rb Human Human, Mouse ELISA, WB, IHC
MIB1 Antibody CSB-PA13289A0Rb Human Human ELISA, WB, IHC, IF
MOV10 Antibody CSB-PA862068LA01HU Human Human ELISA, WB, IHC
MRPS2 Antibody CSB-PA897102LA01HU Human Human ELISA, WB
MRPS5 Antibody CSB-PA014934LA01HU Human Human ELISA, WB
MTCH1 Antibody CSB-PA868369ESR1HU Human Human ELISA, WB, IHC
NARS2 Antibody CSB-PA856950LA01HU Human Human ELISA, IHC
NDUFAF1 Antibody CSB-PA897101LA01HU Human Human ELISA, IHC
NDUFAF2 Antibody CSB-PA836643LA01HU Human Human ELISA, WB, IHC
NDUFB9 Antibody CSB-PA897317LA01HU Human Human, Rat ELISA, WB
NEK9 Antibody CSB-PA837435LA01HU Human Human, Rat ELISA, WB, IHC
NEU1 Antibody CSB-PA17249A0Rb Human Human ELISA, IHC, IF
NIN Antibody CSB-PA843155LA01HU Human Human ELISA, IHC
NINL Antibody CSB-PA896492ESR1HU Human Human ELISA, IHC
NLRX1 Antibody CSB-PA015878LA01HU Human Human ELISA, IHC
NOL10 Antibody CSB-PA015918LA01HU Human Human ELISA, WB, IHC
NPC2 Antibody CSB-PA015976LA01HU Human Human ELISA, IHC, IF
NPTX1 Antibody CSB-PA614901LA01HU Human Human ELISA, IHC, IF
NSD2 Antibody CSB-PA026107LA01HU Human Human ELISA, IHC, IF
NUP210 Antibody CSB-PA016195LA01HU Human Human ELISA, IHC, IF
NUP58 Antibody CSB-PA887135LA01HU Human Human, Mouse ELISA, WB, IHC
NUP62 Antibody CSB-PA016204LA01HU Human Human, Mouse ELISA, WB, IHC, IP
NUP98 Antibody CSB-PA016209LA01HU Human Human ELISA, WB, IF, IP
NUTF2 Antibody CSB-PA016214ESR1HU Human Human ELISA, IHC
OS9 Antibody CSB-PA613402LA01HU Human Human ELISA, WB, IHC, IF
PCSK6 Antibody CSB-PA017645LA01HU Human Human ELISA, IHC
PDE4DIP Antibody CSB-PA716571ESR1HU Human Human ELISA, IHC
PDZD11 Antibody CSB-PA707840HA01HU Human Human ELISA, IHC
PIGO Antibody CSB-PA855068LA01HU Human Human ELISA, IHC, IF
PIGS Antibody CSB-PA847222LA01HU Human Human ELISA, IF
PITRM1 Antibody CSB-PA692283ESR1HU Human Human ELISA, WB, IHC
PKP2 Antibody CSB-PA859116LA01HU Human Human ELISA, IHC
PLAT Antibody CSB-PA11819A0Rb Human Human, Mouse ELISA, WB, IHC
PLD3 Antibody CSB-PA018146LA01HU Human Human ELISA, IHC, IF
PLEKHF2 Antibody CSB-PA884492ESR1HU Human Human ELISA, IHC
PLOD2 Antibody CSB-PA018200LA01HU Human Human ELISA, WB
PMPCA Antibody CSB-PA606021ESR1HU Human Human ELISA, IHC
PMPCB Antibody CSB-PA018243ESR1HU Human Human ELISA, WB, IHC
POLA2 Antibody CSB-PA614517LA01HU Human Human ELISA
POR Antibody CSB-PA06275A0Rb Human Human ELISA, WB, IHC
PPIL3 Antibody CSB-PA863962LA01HU Human Human ELISA, WB, IHC
PPT1 Antibody CSB-PA018587LA01HU Human Human ELISA, WB, IHC
PRIM1 Antibody CSB-PA018680LA01HU Human Human ELISA, IF
PRKAR2A Antibody CSB-PA018697LA01HU Human Human ELISA, WB, IHC, IF
PSMD8 Antibody CSB-PA018913ESR2HU Human Human ELISA, IHC
PTBP2 Antibody CSB-PA892133LA01HU Human Human ELISA, WB
PTGES2 Antibody CSB-PA018977ESR1HU Human Human  ELISA, WB, IHC
PVR Antibody CSB-PA019093ESR1HU Human Human ELISA, WB, IHC
RAB10 Antibody CSB-PA019152LA01HU Human Human ELISA, IHC, IF
RAB18 Antibody CSB-PA878835ESR1HU Human Human ELISA, WB, IHC
RAB1A Antibody CSB-PA00335A0Rb Human Human ELISA, WB
RAB2A Antibody CSB-PA019180LA01HU Human Human ELISA
RAB5C Antibody CSB-PA019215DSR1HU Human Human ELISA, IHC
RAB7A Antibody CSB-PA019219LA01HU Human Human ELISA, IHC, IF
RAE1 Antibody CSB-PA019276ESR1HU Human Human, Mouse ELISA, WB, IHC
RALA Antibody CSB-PA019296LA01HU Human Human ELISA, IHC, IF
RBM41 Antibody CSB-PA850315LA01HU Human Human ELISA, WB, IHC
RBX1 Antibody CSB-PA019496ESR1HU Human Human ELISA, WB, IHC
REEP5 Antibody CSB-PA723351ESR1HU Human Human ELISA, WB, IHC
RHOA Antibody CSB-PA00875A0Rb Human Human ELISA, IHC
RIPK1 Antibody CSB-PA618785LA01HU Human Human, Rat, Mouse ELISA, WB, IF
RNF41 Antibody CSB-PA880976ESR1HU Human Human ELISA, WB, IHC
SCARB1 Antibody CSB-PA845139LA01HU Human Human ELISA, IHC
SDF2 Antibody CSB-PA020900LA01HU Human Human ELISA, IHC
SEPSECS Antibody CSB-PA14389A0Rb Human Human ELISA, WB, IHC, IF
SIGMAR1 Antibody CSB-PA021320LA01HU Human Human ELISA, IHC
SIL1 Antibody CSB-PA875645LA01HU Human Human, Mouse ELISA, WB
SIRT5 Antibody CSB-PA873680LA01HU Human Human ELISA, IHC, IF
SLC27A2 Antibody CSB-PA021534ESR2HU Human Human ELISA
SLC30A6 Antibody CSB-PA744010LA01HU Human Human ELISA, WB, IHC, IF
SLC30A7 Antibody CSB-PA836732LA01HU Human Human, Mouse ELISA, WB, IHC
SLU7 Antibody CSB-PA021783LA01HU Human Human, Mouse ELISA, WB, IHC, IF
SMOC1 Antibody CSB-PA875673LA01HU Human Human, Mouse ELISA, WB, IHC, IF
SRP19 Antibody CSB-PA022674HA01HU Human Human ELISA, WB, IHC
SRP54 Antibody CSB-PA022675LA01HU Human Human, Mouse ELISA, WB, IF
STC2 Antibody CSB-PA022822ESR1HU Human Human ELISA, IHC
STOM Antibody CSB-PA022862ESR1HU Human Human ELISA, WB, IHC
STOML2 Antibody CSB-PA892131ESR1HU Human Human ELISA, IHC
SUN2 Antibody CSB-PA883386LA01HU Human Human, Mouse, Rat ELISA, WB, IHC
TARS2 Antibody CSB-PA023132LA01HU Human Human ELISA, IHC
TBCA Antibody CSB-PA023225HA01HU Human Human ELISA, IHC
TBK1 Antibody CSB-PA023232LA01HU Human Human, Mouse ELISA, WB, IHC, IF
TBKBP1 Antibody CSB-PA023233LA01HU Human Human ELISA, IF
TCEB1 Antibody CSB-PA023281EA01HU Human Human ELISA
TCF12 Antibody CSB-PA023293ESR1HU Human Human ELISA, IHC, IP
THTPA Antibody CSB-PA883600LA01HU Human Human ELISA, IHC
TIMM10 Antibody CSB-PA023548LA01HU Human Human ELISA, IHC
TIMM10B Antibody CSB-PA896532LA01HU Human Human, Mouse ELISA, WB, IHC
TIMM8B Antibody CSB-PA897567LA01HU Human Human ELISA, IHC
TIMM9 Antibody CSB-PA897296LA01HU Human Human ELISA, IHC
TLE1 Antibody CSB-PA717264ESR1HU Human Human ELISA
TM2D3 Antibody CSB-PA863106LA01HU Human Human ELISA, IHC, IF
TOMM70 Antibody CSB-PA024055ESR2HU Human Human ELISA, WB
TOR1A Antibody CSB-PA024067LA01HU Human Human ELISA, IHC, IF
TRIM59 Antibody CSB-PA808999LA01HU Human Human ELISA, IHC
TUBGCP3 Antibody CSB-PA856913ESR2HU Human Human ELISA, IHC
UBAP2L Antibody CSB-PA622754LA01HU Human Human ELISA, IHC, IF
UBXN8 Antibody CSB-PA025539LA01HU Human Human ELISA, IHC
UPF1 Antibody CSB-PA838819LA01HU Human Human ELISA, IF
VPS11 Antibody CSB-PA887959ESR1HU Human Human ELISA, IHC
VPS39 Antibody CSB-PA836235LA01HU Human Human, Mouse ELISA, WB, IHC, IF
ZC3H7A Antibody CSB-PA818245LA01HU Human Human ELISA, IHC
ZDHHC5 Antibody CSB-PA861196LA01HU Human Human ELISA, IHC, IF
  • ELISA试剂盒
靶点 产品货号 种属 样本类型 检测范围
ADAM9 CSB-E11904h Human serum, plasma, tissue homogenates, cell culture supernates, ascitic fluid 62.5 pg/mL-4000 pg/mL
BCS1L CSB-EL002644HU Human serum, plasma, tissue homogenates, cell lysates 7.8 pg/mL-500 pg/mL
BRD4 CSB-EL002802HU Human serum, plasma, tissue homogenates 18.75 pg/mL-1200 pg/mL
COL6A1 CSB-E16375h Human serum, plasma, cell culture supernates, urine 31.25 pg/mL-2000 pg/mL
DNAJC19 CSB-EL007026HU Human serum, plasma, tissue homogenates, cell lysates 28 pg/mL-1800 pg/mL
FBLN5 CSB-EL008454HU Human serum, plasma, cell culture supernates, tissue homogenates 62.5 pg/mL-4000 pg/mL
FBN1 CSB-E13141h Human serum, plasma, tissue homogenates 78 pg/mL-5000 pg/mL
FBN2 CSB-EL008457HU Human serum, plasma, tissue homogenates 0.625 ng/mL-40 ng/mL
GDF15 CSB-E12009h Human serum, plasma, tissue homogenates, urine 7.8 pg/mL-500 pg/mL
GFER  CSB-EL009370HU Human serum, plasma, tissue homogenates, cell lysates 18.75 pg/mL-1200 pg/mL
GGH CSB-EL009389HU Human serum, plasma, tissue homogenates, cell lysates 0.45 ng/mL-30 ng/mL
GLA CSB-E09457h Human serum, plasma, tissue homogenates 7.8 pg/mL-500 pg/mL
GPX1 CSB-EL009866HU Human serum, plasma, tissue homogenates 15.6 μU/mL-1000 μU/mL
HMOX1 CSB-E08266h Human serum, plasma, tissue homogenates 15.6 ng/mL-1000 ng/mL
IL17RA CSB-EL011602HU Human serum, plasma, tissue homogenates, cell lysates 78 pg/mL-5000 pg/mL
IMPDH2 CSB-E17855h Human serum, plasma, cell culture supernates, tissue homogenates 23.5 pg/mL-1500 pg/mL
ITGB1 CSB-EL011880HU Human serum, plasma, tissue homogenates 62.5 pg/mL-4000 pg/mL
LOX CSB-E16376h Human serum, plasma, tissue homogenates 0.625 ng/mL-40 ng/mL
MFGE8 CSB-E12637h Human serum, cell culture supernates, urine, cerebrospinal fluid (CSF) 781.25 pg/mL-50000 pg/mL
NEU1 CSB-E13811h Human serum, urine, cerebrospinal fluid (CSF) 0.312 ng/mL-20 ng/mL
NPC2 CSB-EL015976HU Human serum, plasma, tissue homogenates, cell lysates 0.312 ng/mL-20 ng/mL
NPTX1 CSB-E14336h Human serum, plasma 7.8 pg/mL-500 pg/mL
PLAT CSB-E07916h Human serum, palsma, tissue homogenates.  0.156 ng/ml-10 ng/ml
PLOD2 CSB-EL018200HU Human serum, plasma, tissue homogenates 9.375 pg/mL-600 pg/mL
PVR CSB-EL019093HU Human serum, plasma, tissue homogenates 7.8 ng/mL-500 ng/mL
RAB10 CSB-EL019152HU Human serum, plasma, tissue homogenates, cell lysates 39 pg/mL-2500 pg/mL
RTN4 CSB-EL020572HU Human serum, plasma, tissue homogenates 46.88 pg/mL-3000 pg/mL
SELENOS CSB-EL027265HU Human serum, plasma, tissue homogenates, cell lysates 31.25 pg/mL-2000 pg/mL
SIGMAR1 CSB-EL021320HU Human serum, plasma, tissue homogenates, cell lysates 0.156 ng/mL-10 ng/mL
SMOC1 CSB-EL021842HU Human serum, plasma, tissue homogenates 78 pg/mL-5000 pg/mL
STC2 CSB-EL022822HU Human serum, plasma, tissue homogenates, cell lysates 31.2 pg/mL-2000 pg/mL

References

[1] XuhuaZhang, XiaodongLv, et al. Short-term culture with IL2 is beneficial for potent miory chimeric antigen receptor T cell production [J]. Biochiical and Biophysical Research Communications. 2018, 495(2): 1833-1838.

[2] Craig T. Lefort and Minsoo Kim. Human T Lymphocyte Isolation, Culture and Analysis of Migration [J]. J Vis Exp. 2010; (40): 2017.

[3] P Choi and H Reiser. IL4: role in disease and regulation of production [J]. Clin Exp Immunol. 1998 Sep; 113(3): 317–319.

[4] Chavez, A. R., Buchser, W., et al. Ann. N.Y. Acad. Sci. 2009, 1182, 14-27.

[5] Scott Wilkie, et al. Selective Expansion of Chimeric Antigen Receptor-targeted T-cells with Potent Effector Function using Interleukin-4 [J]. JOURNAL OF BIOLOGICAL CHiISTRY. 2010; 285(33):25538-25544.

[6] Shanafelt, Forte, C. P., et al. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 9454-9458.

[7] Saba Ghassii, Felipe Bedoya, et al. Shortened T Cell Culture with IL7 and IL15 Provides the Most Potent Chimeric Antigen Receptor (CAR)-Modified T Cells for Adoptive Immunotherapy [J]. J Immunol. 2016, 196.

[8] Alizadeh D,Wong RA, et al. IL15 Enhances CAR-T Cell Antitumor Activity by Reducing mTORC1 Activity and Preserving Their Sti Cell Miory Phenotype [J]. Cancer Immunol Res. 2019 7(5):759-772.

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