COVID-19 Antibody Reagents

SARS-CoV-2抗体

新冠病毒引起的新冠肺炎疫情此起彼伏,对全球的医疗卫生系统和经济带来了巨大影响,同时也给新冠疫苗、治疗药物以及诊断带来了新的挑战。武汉华美生物一直密切关注新冠病毒的进化情况,目前已开发了大量高质量SARS-CoV-2相关抗体试剂,包括N抗体、S抗体、S RBD纳米抗体、N抗体对和S RBD抗体对等。

其中,N抗体与S抗体可用于新冠病毒致病性机理研究,可通过WB定性检测新冠抗原;N抗体对和S RBD抗体对可用于新冠试剂盒的开发;另外,部分抗体验证了中和试验和主流突变株检测,可作为质控品,应用于针对突变株的中和抗体筛选、重组蛋白疫苗研发、疫苗接种后血清中的中和抗体检测等

华美特色

  • 抗体试剂类型多
  • 高特异性
  • 多种应用场景验证
  • 多个主流突变株验证

部分应用场景及验证数据

● WB定性抗原检测

Positive WB detected in: His tag-tagged SARS-CoV-2 N recombinant protein (CSB-EP3325GMY); SARS-CoV-2 N antibody at 1:1000 (CSB-RA33255A1GMY)

Positive WB detected in: SARS-CoV-2 N recombinant protein (CSB-EP3325GMY) at 40ng, 20ng, 10ng; All lanes N antibody (CSB-MA33255A0m) at 1:1000

● 功能性ELISA验证抗原-抗体结合活性

Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/mL can bind SARS-CoV-2-N (CSB-RA33255A0GMY) Antibody, the EC50 is 6.892 ng/mL.

Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-MA33245A0m), the EC50 is 32.59 to 39.33 ng/mL.

● 用于胶体金(GICA)快速检测

In the GICA detection system, the background of antibody (CSB-RA33255A2GMY) is clean, the detection limit can be as low as 15.625ng/mL (1.09ng/0.07mL), and the sensitivity is very good.

In the GICA detection system, the background of antibody (CSB-RA33245A0GMY) is clean, the detection limit can be as low as 13.94ng/mL (0.976ng/0.07mL), and the sensitivity is very good.

● 抗体对经S-ELISA验证

SARS-CoV-2 N Antibody Pair 1 (CSB-EAP33255A1) is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA).

SARS-CoV-2 Spike RBD Antibody Pair 1 (CSB-EAP33245) is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA).

● 经中和试验验证中和抗体

Binding signal of SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) and ACE2 protein-HRP conjugate (CSB-MP866317HU) was inhibited by S Antibody (CSB-RA33245A1GMY) with the IC50 is 2.38 μg/mL

Binding signal of SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) and ACE2-HRP (CSB-YP3324GMY1) was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the IC50 is 0.1074 μg/mL.

● 多个突变株识别验证

Protein Code Variants EC50 (ng/mL)
CSB-EP3325GMY(M1) D3L, R203K, G204R, S235F 2. 562-3.421
CSB-EP3325GMY(M2) T205I 29.34-40.67
CSB-EP3325GMY(M3) P80R 63.78-113.6
CSB-EP3325GMY(M4) P13L 2.825-4.124
CSB-EP3325GMY(M5) D103Y 7.358-9.976
CSB-EP3325GMY(M8) S202N 1.466-2.382
CSB-EP3325GMY(M9) L230F 4.854-7.721
CSB-EP3325GMY(M10) I292T 3.859-7.358
CSB-EP3325GMY(M11) Q384H 2.785-4.100
CSB-EP3325GMY(M12) R203K, G204R 4.664-8.256
CSB-EP3325GMY(M13) R203M, D377Y 0.8488-1.385
CSB-EP3325GMY(M14) D63G, R203M, D377Y 1.304-1.875

The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/mL can bind N Recombinant Antibody (CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody.

Protein Code Variants EC50 (ng/mL)
CSB-MP3324GMY1(M8) E484K 371.0-487.5
CSB-MP3324GMY1(M7) K417N 4.522-5.950
CSB-MP3324GMY1(M6) N501Y 47.09-86.25
Protein Code Variants EC50 (ng/mL)
CSB-MP3324GMY1(M9) K417T 8.706-13.61
CSB-MP3324GMY1(M10) K417N, E484K, N501Y 97.92-169.6
CSB-MP3324GMY(M1) D614G 23.19-34.02

The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/mL can bind S Recombinant Antibody, the EC50 of S Recombinant Antibody (CSB-RA33245A1GMY).

产品列表

● N antibody

货号 产品名称 反应种属 应用类型
CSB-RA33255A1GMY N Antibody SARS-CoV-2 ELISA, WB, GICA
CSB-RA33255A2GMY N Antibody SARS-CoV-2 ELISA
CSB-MA33255A0m N Antibody GICA Mouse
CSB-PA33254A0Rb N Antibody GICA Rabbit
CSB-RA33255A0GMY N Antibody SARS-CoV-2 ELISA, GICA
CSB-RA33255B1GMY N Antibody, HRP conjugated SARS-CoV-2 ELISA
CSB-RA33255C1GMY N Antibody, FITC conjugated SARS-CoV-2 ELISA
CSB-RA33255D1GMY N Antibody, Biotin conjugated SARS-CoV-2 ELISA
CSB-MA33255A1m N Antibody GICA Mouse
CSB-MA33255A2m N Antibody GICA Mouse
CSB-PA33254B0Rb N Antibody, HRP conjugated SARS-CoV-2 ELISA
CSB-PA33254C0Rb N Antibody, FITC conjugated SARS-CoV-2 ELISA
CSB-PA33254D0Rb N Antibody, Biotin conjugated SARS-CoV-2 ELISA

● S antibody

货号 产品名称 反应种属 应用类型
CSB-RA33245A1GMY S Antibody SARS-CoV-2 ELISA, GICA, Neutralising
CSB-MA33245A2m S Monoclonal Antibody GICA Mouse
CSB-PA33245YA01GMY S Antibody GICA Rabbit
CSB-RA33245A0GMY S Antibody SARS-CoV-2 ELISA, GICA
CSB-RA33245B1GMY S Antibody, HRP conjugated SARS-CoV-2 ELISA
CSB-RA33245C1GMY S Antibody, FITC conjugated SARS-CoV-2 ELISA
CSB-RA33245D1GMY S Antibody, Biotin conjugated SARS-CoV-2 ELISA
CSB-MA33245A0m S Monoclonal Antibody GICA Mouse
CSB-MA33245A1m S Monoclonal Antibody GICA Mouse
CSB-PA33245YB01GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YC01GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YD01GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YA11GMY S Antibody GICA Rabbit
CSB-PA33245YB11GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YC11GMY S Antibody SARS-CoV-2 ELISA
CSB-PA33245YD11GMY S Antibody SARS-CoV-2 ELISA

● N Antibody Pair

货号 产品名称 反应种属 应用类型
CSB-EAP33255A2 SARS-CoV-2 N Antibody Pair 2 SARS-CoV-2 S-ELISA
CSB-EAP33255A1 SARS-CoV-2 N Antibody Pair 1 SARS-CoV-2 S-ELISA
CSB-EAP33255A3 SARS-CoV-2 N Antibody Pair 3 SARS-CoV-2 S-ELISA
CSB-EAP33255A4 SARS-CoV-2 N Antibody Pair 4 SARS-CoV-2 S-ELISA

● S RBD Nanobody Antibody

货号 产品名称 反应种属 应用类型
CSB-RA33245A2GMY SARS-CoV-2 RBD Nanobody SARS-CoV-2 ELISA, GICA, Neutralising
CSB-RA33245B2GMY SARS-CoV-2 RBD Nanobody, HRP conjugated SARS-CoV-2 ELISA
CSB-RA33245C2GMY SARS-CoV-2 RBD Nanobody, FITC conjugated SARS-CoV-2 ELISA
CSB-RA33245D2GMY SARS-CoV-2 RBD Nanobody, Biotin conjugated SARS-CoV-2 ELISA

● S RBD Antibody Pair

货号 产品名称 反应种属 应用类型
CSB-EAP33245 SARS-CoV-2 Spike RBD Antibody Pair 1 SARS-CoV-2 S-ELISA