Recombinant Mouse LIM domain-containing protein ajuba(Ajuba)

  • 货号:
    CSB-YP856315MO
  • 规格:
  • 来源:
    Yeast
  • 其他:
  • 货号:
    CSB-EP856315MO
  • 规格:
  • 来源:
    E.coli
  • 其他:
  • 货号:
    CSB-EP856315MO-B
  • 规格:
  • 来源:
    E.coli
  • 共轭:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 货号:
    CSB-BP856315MO
  • 规格:
  • 来源:
    Baculovirus
  • 其他:
  • 货号:
    CSB-MP856315MO
  • 规格:
  • 来源:
    Mammalian cell
  • 其他:

产品详情

  • 纯度:
    >85% (SDS-PAGE)
  • 基因名:
    Ajuba
  • Uniprot No.:
  • 别名:
    Ajuba; Jub; LIM domain-containing protein ajuba
  • 种属:
    Mus musculus (Mouse)
  • 蛋白长度:
    full length protein
  • 表达区域:
    1-547
  • 氨基酸序列
    MERLGEKASR LLEKLRLSDS GSAKFGRRKG EASRSGSDGT PGAGKGRLSG LGGPRKSGHR GANGGPGDEP LEPAREQGPL DAERNARGSF EAQRFEGSFP GGPPPTRALP LPLSSPPDFR LETTAPALSP RSSFASSSAS DASKPSSPRG SLLLDGAGAS GAGGSRPCSN RTSGISMGYD QRHGSPLPAG PCLFGLPLTT APAGYPGGAP SAYPELHAAL DRLCAHRSVG FGCQESRHSY PPALGSPGAL TGAVVGTAGP LERRGAQPGR HSVTGYGDCA AGARYQDELT ALLRLTVATG GREAGARGEP SGIEPSGLEE SPGPFVPEAS RSRIREPEAR EDYFGTCIKC NKGIYGQSNA CQALDSLYHT QCFVCCSCGR TLRCKAFYSV NGSVYCEEDY LFSGFQEAAE KCCVCGHLIL EKILQAMGKS YHPGCFRCIV CNKCLDGVPF TVDFSNQVYC VTDYHKNYAP KCAACGQPIL PSEGCEDIVR VISMDRDYHF ECYHCEDCRM QLSDEEGCCC FPLDGHLLCH GCHMQRLSAR QPSTNYI
  • 蛋白标签:
    The following tags are available.
    N-terminal His-tagged
    Tag-Free
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式:
    Lyophilized powder
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

靶点详情

  • 功能:
    Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.
  • 基因功能参考文献:
    1. Ajuba recruits p300/CBP via its LIM domain and facilitates p300/CBP binding to PPARg. Moreover, Ajuba, PPARg, p300/CBP can cooperatively occupy the PPARg target promoters and concomitantly increases histone acetylation at these loci. PMID: 26113042
    2. Ajuba is a novel coactivator for liver X receptors and may play important role in lipid and glucose metabolism. PMID: 26389695
    3. Findings support the importance of adhesion molecules (VE-cadherin and CD31), survivin, and Ajuba in modulating the Hippo pathway, which regulates, in part, proliferation and survival in hemangioendotheliomas. PMID: 25266662
    4. PKD1-mediated phosphorylation of SNAI1 occurs in the nucleus and generates a nuclear, inactive DNA/SNAI1 complex that shows decreased interaction with its co-repressor Ajuba. PMID: 22276203
    5. This paper presents evidence indicating that the human and mouse Ajuba is a new cytosolic component of the IL-1 signaling pathway, influencing the assembly and activity of the aPKC/p62/TRAF6 multiprotein signaling complex. PMID: 15870274
    6. identification of the protein arginine methyltransferase 5 (PRMT5) as an effector recruited to SNAIL through an interaction with AJUBA that functions to repress the SNAIL target gene, E-cadherin PMID: 18347060

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  • 亚细胞定位:
    Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, P-body.
  • 蛋白家族:
    Zyxin/ajuba family
  • 组织特异性:
    Expressed in skin, brain and genitourinary organs.
  • 数据库链接: