Recombinant Mouse Caspase-1(Casp1) ,partial

  • 货号:
    CSB-YP004543MO
  • 规格:
  • 来源:
    Yeast
  • 其他:
  • 货号:
    CSB-EP004543MO
  • 规格:
  • 来源:
    E.coli
  • 其他:
  • 货号:
    CSB-EP004543MO-B
  • 规格:
  • 来源:
    E.coli
  • 共轭:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 货号:
    CSB-BP004543MO
  • 规格:
  • 来源:
    Baculovirus
  • 其他:
  • 货号:
    CSB-MP004543MO
  • 规格:
  • 来源:
    Mammalian cell
  • 其他:

产品详情

  • 纯度:
    >85% (SDS-PAGE)
  • 基因名:
    Casp1
  • Uniprot No.:
  • 别名:
    Casp1; Il1bcCaspase-1; CASP-1; EC 3.4.22.36; Interleukin-1 beta convertase; IL-1BC; Interleukin-1 beta-converting enzyme; ICE; IL-1 beta-converting enzyme; p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10]
  • 种属:
    Mus musculus (Mouse)
  • 蛋白长度:
    Partial
  • 表达区域:
    -
  • 蛋白标签:
    The following tags are available.
    N-terminal His-tagged
    Tag-Free
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式:
    Lyophilized powder
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

靶点详情

  • 功能:
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis (By similarity). Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive
  • 基因功能参考文献:
    1. ABT-737 elicited an anti-atopic dermatitis (AD) activity via suppression of caspase-1 activation in AD in vitro and in vivo models. Therefore, this study provides important information regarding the use of anticancer drugs for controlling allergic inflammatory diseases. PMID: 29957081
    2. Casp1Null mice expressed caspase-11 proteins and did not secrete IL-1beta and IL-18 or undergo pyroptosis in response to canonical NLRP3, NLRC4 or AIM2 inflammasome activators. PMID: 28345580
    3. The enzymatic activities of caspase-1 and caspase-11 are required for growth inhibition in different cell types.These results reveal that these proteases have important functions beyond the direct induction of pyroptosis and proinflammatory cytokine secretion in the control of growth and elimination of cytosolic bacteria. PMID: 27808091
    4. Collectively, these results are consistent with a model whereby the type III secretion system apparatus of Pseudomonas aeruginosa activates the caspase-1-dependent inflammasome and caspase-3/7 through an ASC-dependent mechanism. PMID: 29957172
    5. The results of this study indicated that beta-Amyloid peptide-induced caspase-1 activation, disrupts autophagy in the cortex and in the hippocampus resulting in neurodegeneration and memory loss. PMID: 28801921
    6. caspase-1 regulates dopaminergic neuronal death in the pathogenesis of Parkinson's disease in mice via caspase-7/PARP1/AIF pathway. PMID: 27339879
    7. RIP3-mediated activation of caspase-1 rather than necroptosis-dependent inflammation was responsible for aggressive inflammation in influenza A (H7N9) virus-infected mice. PMID: 28423682
    8. we assessed the role of RIP3 in synergy with Caspase-1 in the induction of IL-1beta production in BMDM after either LPS/ATP or Chlamydia muridarum stimulation. The possibility of pyroptosis and necroptosis interplays and the role of RIP3 in IL-1beta production during Chlamydia muridarum infection in BMDM was investigated as well. PMID: 28660207
    9. Studied the role of transient receptor potential melastatin 2 (TRPM2) in activating caspase-1 and caspase-1-dependent pyroptosis in mouse BMDMs. Found TRPM2 knockout caused higher caspase-1 activation and pyrotopsis. PMID: 29250536
    10. The results demonstrate the activation of the caspase 1 precursor by caspase 11 and suggest a new mechanism of protection of Theiler murine encephalomyelitis virus-infected astrocytes from apoptosis. PMID: 26567013
    11. Nodakenin inhibited the mRNA expression and production of pro-inflammatory cytokines and caspase-1 activation PMID: 28407357
    12. Adult neural stem cells (ANSCs) expressed NLRP3-containing inflammasome and alpha-syn activated both TLR4/NF-kappaB and NLRP3/caspase-1 signals in ANSCs. PMID: 26676570
    13. dimerized or endogenous caspase-8 can also directly cleave IL-1beta into its biologically active form, in the absence of canonical inflammasome components. PMID: 27419363
    14. Report direct role of pleural cells in the pathogenesis of bleomycin-induced pulmonary fibrosis via caspase-1/IL-1beta pathway. PMID: 27894300
    15. caspase-1-/- mice exhibited resistance to indomethacin-induced small intestinal damage PMID: 26349656
    16. data provide evidence that the NLRC4 inflammasome contributes to resistance through regulation of caspase-1, IL-1beta and IL-18 in a CD11blowLy6Glow population of cells PMID: 28961278
    17. Hemorrhagic shock primes for lung vascular endothelial cell pyroptosis following lipopolysaccharide exposure through TLR4, which activates Nlrp3, and subsequently induces caspase-1 activation. PMID: 27607578
    18. The most well-known function of active caspase-1 is to cleave the proforms of inflammatory cytokines IL-1beta and -18 into their active forms in response to inflammatory stimuli in immune cells. Caspase-1 has multiple functions in addition to this cytokine maturation role. It is at the center of many cell responses to stress and inflammation not just in immune cells but in other cell types, such as epithelia. Review. PMID: 27450556
    19. Findings demonstrate a critical role of caspase-1 in macrophage-driven inflammation in the adipose tissue and the development of obesity. PMID: 27702746
    20. Inflammasome NLRP3-caspase-1-mediated degradation of smooth muscle cell contractile proteins may contribute to aortic biomechanical dysfunction and aortic aneurysm/dissection development. PMID: 28153878
    21. Data indicate that NLRC4 activation in Intestinal epithelial cells (IECs) leads to cell expulsion and IL-18 release, and implicate Caspase-8 in NLRC4 inflammasome responses in vivo by generation of doubly deficient in Caspase-1 and Caspase-8. PMID: 28410991
    22. our studies have uncovered a specific role for caspase-1-mediated IL-1beta release in the manifestation of Familial Mediterranean Fever PMID: 27998728
    23. variant p.C284A caspase-1 stabilizes pyroptosome formation, potentially enhancing inflammation by two IL-1beta-independent mechanisms: pyroptosomes convey an enhanced inflammatory stimulus through the recruitment of additional proteins (such as RIP2, receptor interacting protein kinase 2), which is further amplified through pyroptosome and cell division. PMID: 27402835
    24. TNF-alpha induces caspase-1 activation in an inflammasome-independent manner in 3T3-L1 cells and that the ERK-dependent expression of NLRP3 may play a role independently of its canonical role as a component of inflammasomes. PMID: 26989816
    25. caspase-1 activation is an upstream event of apoptotic caspase-7 induction in renal tubulointerstitial fibrosis PMID: 27643624
    26. it was found that GSK2656157 specifically inhibited ER stress induced by large amount of LPS and reduced LPS-induced IL-1beta production through inhibition of Caspase 1 activation. PMID: 27251848
    27. Study suggests that Casp1 has a limited role in the development of burn-induced pain. PMID: 28040362
    28. Data show that inhibiting caspase-1 activity in the NLR family, pyrin domain-containing 3 protein (NLRP3) inflammasome decreased gastric cell apoptosis. PMID: 27053298
    29. Chronic kidney disease-mediated increase of contractile markers in vascular smooth muscle cells and increased expression of VSMC migration molecule alphavbeta3 integrin in NH formation were caspase-1 dependent. PMID: 26928596
    30. Demonstrate the utility of intracellular caspase 1 and extracellular CTSS proteolytic activities as surrogate biomarkers of lysosomal rupture and acute inflammation. PMID: 27108091
    31. Caspase-1-/- mice have intact immune responses but do not develop an expanded marginal zone B cell population in response to pristane-induced lupus. This may be one explanation for reduced IgG autoantibody production in these mice. PMID: 26405027
    32. study uncovered an important role for YopJ in inhibiting caspase-1 in activated macrophages and in promoting Yersinia virulence PMID: 26810037
    33. Caspase-1-activated IL-18 induces IFN-gamma to prime caspase-11 and rapidly clear Burkholderia thailandensis infection. PMID: 26320999
    34. These results argue that caspase-1, -4 or -5 can be recruited to inflammasomes under specific circumstances, often leading to distinctly organized and localized complexes that may impact the functions of these proteases PMID: 26158519
    35. Prophylactic treatment with capsase-1 inhibitor VX756 ameliorated the severity and progression of collagen-induced arthritis. PMID: 26742834
    36. The resilience of casp1(-/-) mice to SIRS lethality is associated with a lower inflammatory response, loss of hippocampal gene coexpression patterns, and increased hippocampal Adamts1 gene expression. PMID: 25633245
    37. Internalized Cryptococcus neoformans Activates the Canonical Caspase-1 and the Noncanonical Caspase-8 Inflammasomes. PMID: 26466953
    38. This study highlights a critical role of caspase-1 activation and NLPR3/ASC inflammasome complex in the down-modulation of IL-33 in vivo and in vitro. PMID: 25714839
    39. Data show that caspase-1 but not caspase-11 was essential for the functional activities of the NLRC4 inflammasome. PMID: 26232428
    40. Data indicate that genetic ablation of caspase-1 and -11 from cpdm mice significantly reduced skin inflammation in sharpin-deficient mice. PMID: 26216893
    41. In an inflammatory microenvironment, caspase-1 is regulated by NF-kappaB. PMID: 25815426
    42. ASC-driven caspase-1 autoprocessing and speck formation are dispensable for the activation of caspase-1 and the NLRP1b inflammasome. PMID: 24492532
    43. The signalling pathway governing the inflammasome formation in the heart requires a priming signal in order for an active NLRP3 to induce caspase-1 activation PMID: 25524927
    44. GATA4, a key regulator of cardiomyocyte survival, is a substrate for caspase-1. PMID: 25501827
    45. Data (including data from studies in knockout mice) suggest that caspase-1/11 are involved in development of atherosclerosis; hematopoietic deletion of caspase-1/11 results in strong reduction in atherosclerotic plaque size. PMID: 25817537
    46. There may be a protective role of caspase-1 in keratinocytes during UVB-induced skin cancer development through the induction of apoptosis. PMID: 25562666
    47. These findings suggest that endogenous caspase-1/11 activity, independent of the NLRP3 inflammasome, not only promotes the magnitude of the inflammatory response, but also suppresses protective immunity in the neonate PMID: 25684123
    48. Data suggest that Casp1 is activated and expression/nucleocytoplasmic translocation of Hmgb1 (high mobility group box 1) is up-regulated in pulmonary mucosa in asthma; these changes involve PI3K (phosphatidylinositol 3-kinase) signal pathway. PMID: 25929181
    49. Inflammasome-induced activation of an intracellular caspase-1/calpain cysteine protease cascade degraded filamin. Caspase-1 specifically facilitated actin exposure, which released of highly procoagulant MPs from filopodia. PMID: 25705884
    50. caspase-1/ASC inflammasomes play a significant role in the activation of IL-1beta/ROS and NF-kappaB signaling of cytokine gene expression for T. cruzi control in human and mouse macrophages. PMID: 25372293

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  • 亚细胞定位:
    Cytoplasm
  • 蛋白家族:
    Peptidase C14A family
  • 组织特异性:
    High level expression seen in spleen and lung, low level expression seen in brain, heart, liver, kidney, testis and skeletal muscle.
  • 数据库链接: