N Antibody

  • 货号:
  • 规格:
  • 图片:
    • Western Blot
      Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
      SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
      Peroxidase-Affinipure Goat Anti-Human IgG Fcγ Fragment Specific at 1/20000 dilution
      Predicted band size: 48 kDa
      Observed band size: 55 kDa
    • The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
      Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml.
    • In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.
    • ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.
  • 其他:


  • 产品描述:

    Recombinant anti-SARS-CoV-2 Nucleoprotein (N protein) Mouse single-chain variable fragment (ScFv) is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. The resulting product is the recombinant SARS-CoV-2 N monoclonal antibody. This N antibody is recommended for ELISA, WB, and GICA applications. It has undergone affinity-chromatography purification. And it can detect the endogenous content of human SARS-Cov-2 N protein.

    The SARS-CoV-2 N protein is an abundant RNA-binding protein. It contains two structured domains: the RNA binding domain and the oligomerization domain, which are separated by a long flexible linker. The separated domains allow the N protein to execute many functions in the viral lifecycle such as RNA replication, virion assembly, and immune system interference.

  • Uniprot No.:
  • 别名:
    Nucleocapsid protein, NC, protein N, N
  • 反应种属:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 免疫原:
    Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
  • 免疫原种属:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 标记方式:
  • 克隆类型:
  • 抗体亚型:
    Mouse scFv fusion with human IgG1 Fc
  • 纯化方式:
  • 克隆号:
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式:
  • 应用范围:
  • 推荐稀释比:
    Application Recommended Dilution
    ELISA 1:10000-1:50000
    WB 1:500-1:5000
    GICA 1:1000-1:10000
  • Protocols:
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.