Recombinant Human Novel Coronavirus Nucleoprotein(N)

In Stock
  • 货号:
    CSB-EP3325GMY
  • 规格:
  • 图片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    • Western Blot
      Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
      SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
      Secondary
      Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific(CSB-PA160506) at 1/20000 dilution
      Predicted band size: 48 kDa
      Observed band size: 55 kDa

    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A1GMY), the EC50 of SARS-CoV-2-N protein is 1.368 -1.804 ng/ml.

      Biological Activity Assay

    • Activity
      Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A0GMY), the EC50 of SARS-CoV-2-N protein is 4.267-5.568 ng/ml.

      Biological Activity Assay

  • 其他:

产品详情

  • 纯度:
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性:
    ①Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A1GMY), the EC50 of SARS-CoV-2-N protein is 1.368 -1.804 ng/ml. ②Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody (CSB-RA33255A0GMY), the EC50 of SARS-CoV-2-N protein is 4.267-5.568 ng/ml.
  • Uniprot No.:
  • 别名:
    Nucleocapsid protein, NC, protein N, N
  • 种属:
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 蛋白长度:
    Full Length
  • 来源:
    E.coli
  • 分子量:
    48.0kDa
  • 表达区域:
    1-419aa
  • 氨基酸序列
    MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTAAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
  • 蛋白标签:
    N-terminal 6xHis-tagged
  • 产品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • 产品描述:
    Recombinant Human Novel Coronavirus Nucleoprotein (N) (SARS-CoV-2) is a full length protein expressed with an N-terminal 6xHis-tagged in the E.coli. Its expression region corresponds to 1-419aa of Human Novel Coronavirus Nucleoprotein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band of 51.0 kDa on the gel. It was validated to be biologically active through WB and functional ELISA analyses. This recombinant SARS-CoV-2 N proteins in-stock are available.
    N protein is encoded by the SARS-CoV-2 genome. It is highly immunogenic and abundantly expressed during infection. And it is often used in vaccine development and serological assay. After infection, the N protein together with the viral RNA is released into the host cell, promoting viral RNA package, viral particle assembly and release.
  • Datasheet & COA:
    Please contact us to get it.