Rat luteinizing hormone (LH) Elisa kit
Datasheet-
中文名称:大鼠黄体生成素(LH)ELISA kit
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货号:CSB-E12654r
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规格:96T
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价格:3600
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其他:
产品详情
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别名:LhbLutropin subunit beta ELISA Kit; Lutropin beta chain ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit
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缩写:LH
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Uniprot No.:
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma
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检测范围:0.3 mLU/mL-60 mLU/mL
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灵敏度:0.15 mLU/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Signal Transduction
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 88-100 1:2 Average % 95 Range % 91-101 1:4 Average % 94 Range % 85-105 1:8 Average % 96 Range % 90-102 -
回收率:
The recovery of rat LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 98 93-107 EDTA plasma (n=4) 96 91-103 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. mIU/ml OD1 OD2 Average 60 0.150 0.153 0.152 15 0.352 0.338 0.345 4.8 0.513 0.549 0.531 1.2 0.985 1.002 0.994 0.3 1.476 1.441 1.459 0 2.679 2.654 2.667 -
货期:3-5 working days
引用文献
- Umbelliferone Inhibits Spermatogenic Defects and Testicular Injury in Lead-Intoxicated Rats by Suppressing Oxidative Stress and Inflammation, and Improving Nrf2/HO-1 Signaling MF Alotaibi,Drug Des Devel Ther,2020
- Effect of Vitex Agnus-Castus Ethanolic Extract on Sex Hormones in Streptozotocin-Induced Diabetic Rats F Soleymanzadeh,Journal of Family and Reproductive Health,2020
- D-ribose-L-cysteine improves hormonal imbalance, spermatogenic dysregulation, and redox status in streptozotocin-diabetic rats VO Ukwenya,Comparative Clinical Pathology,2020
- The protective role of l-carnitine on spermatogenesis after cisplatin treatment during prepubertal period in rats: A pathophysiological study AM Aboul-Naga,Life Sciences,2020
- Continuous Light-Induced PCOS-Like Changes in Reproduction, Metabolism, and Gut Microbiota in Sprague-Dawley Rats W Chu,Front. Microbiol,2020
- Recombinant B2L and Kisspeptin-54 DNA Vaccine Induces Immunity Against Orf Virus and Inhibits Spermatogenesis In Rats Wassie T, et al,Scientific Reports,2019
- PROTECTIVE EFFECTS OF CACTUS AND/OR PAPAYA JUICES AGAINST HEPATIC AND TESTICULAR TOXICITY INDUCED BY CHLORPYRIFOS IN ALBINO RATS Mogahed H E, et al,World Journal of Pharmaceutical Research,2019
- Traditional Medicine (Mahuang-Tang) Improves Ovarian Dysfunction and the Regulation of Steroidogenic Genes in Letrozole-Induced PCOS Rats Yang H, et al,Journal of Ethnopharmacology,2019
- The Effect of Hydroalchoholic Extract of Cinnamon zeylanicum on Oxidative Damages and Biochemical Change in Adult Rats With Polycystic Ovary Syndrome Khodaeifar F, et al,Crescent Journal of Medical and Biological Sciences,2019
- Effects of Osteocalcin on Synthesis of Testosterone and INSL3 during Adult Leydig Cell Differentiation Gulfidan Coskun, et al,International Journal of Endocrinology,2019
- Comparative Studies on Chemical Contents and Effect in Kidney-Yang Deficiency Rats of Salt-Processed Product and Wine-Processed Product of Cuscutae Semen Ying Zhang, et al,Evidence-Based Complementary and Alternative Medicine,2019
- Abnormal expression of HSP70 may contribute to PCOS pathology Wu G, et al,Journal of Ovarian Research,2019
- Crotonaldehyde-induced alterations in testicular enzyme function and hormone levels, and apoptosis in the testes of male Wistar rats are associated with oxidative damage Zhang B, et al,Toxicology Mechanisms and Methods,2019
- REPRODUCTIVE AGING OF HYPOTHALAMUS-PITUITARY-TESTICULAR AXIS IN MIDDLE-AGED MALE RATS Patteera Wititsuwankul, et al,Oxidative Medicine and Cellular Longevity,2018
- Differential effect of Taraxacum officinale L. (dandelion) root extract on hepatic and testicular tissues of rats exposed to ionizing radiation Abdel-Magied N, et al,Molecular Biology Reports,2019
- The Effects of Anacyclus pyrethrum Alcoholic Root Extract on FSH, LH, Testosterone and Sperm Count in Diabetic Male Rats Mohammad Reza Shahraki,Zahedan Journal of Research In Medical Sciences,2019
- Protective effects of tribulus terrestris extract and angiotensin blockers on testis steroidogenesis in copper overloaded rats Arafa MH, et al,Ecotoxicology and Environmental Safety,2019
- Effects of soya-milk on reproductive hormones during puberty in male Wistar rats Caceres S, et al,reproduction in domestic animals,2019
- The Effect of Electromagnetic Fields of Extremely Low Frequency 30 Hz on Rat Ovaries Alekperov SI, et al,Bulletin of Experimental Biology and Medicine,2019
- Impact of Hydroalcoholic Extract of Humulus Lupulus L. on Sperm Quality, Reproductive Organs and Hormones in Male Rat Karbalaei N, et al,Chinese journal of integrative medicine,2019
- Impact of Sleep Deprivation on the Hypothalamic-Pituitary-Gonadal Axis and Erectile Tissue Lee DS, et al,Journal of Sexual Medicine,2019
- Protective role of Ficus deltoidea against BPA-induced impairments of the follicular development, estrous cycle, gonadotropin and sex steroid hormones level of prepubertal rats Zaid SSM, et al,Journal of ovarian research,2018
- Cyanidin-3-O-glucoside at Low Doses Protected against 3-Chloro-1, 2-propanediol Induced Testis Injury and Improved Spermatogenesis in Male Rats Jiang X, et al,Journal of Agricultural and Food Chemistry,2018
- Crataegus aronia enhances sperm parameters and preserves testicular architecture in both control and non-alcoholic fatty liver disease-induced rats Dallak M, et al,Pharmaceutical Biology,2018
- Amelioration of diabetes-\induced testicular and sperm damage in rats by cerium oxide nanoparticle treatment Tayebe Artimani.et al,ANDROLOGIA,2018
- Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring Hassan I.H.El-Sayyad.et al,Reproductive Biology,2018
- The Effect of Galega officinalis on Hormonal and Metabolic Profile in a Rat Model of Polycystic Ovary Syndrome Seyed Hosein Abtahi-Eivari.et al,International Journal of Women?? Health and Reproduction Sciences,2018
- TOXIC EFFECTS OF TRAZADONE ON MALE REPRODUCTIVE SYSTEM VIA DISRUPTING HYPOTHALAMIC-PITUITARY-TESTICULAR AXIS AND INDUCING TESTICULAR OXIDATIVE STRESS Sinem Ilgin.et al,/,2018
- Tetragonia tetragonioides (Pall.) Kuntze Regulates Androgen Production in a Letrozole-Induced Polycystic Ovary Syndrome Model Bo-Jeong Pyun.et al,Molecules,2018
- Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury Mohammadreza Gholami .et al,Journal of Complementary and Integrative Medicine,2018
- Fructus Ligustri Lucidi modulates estrogen receptor expression with no uterotrophic effect in ovariectomized rats Tang YQ.et al,BMC Complement Altern Med,2018
- Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats Ivana Jari?.et al,Toxicology and Applied Pharmacology,2018
- Lactational exposure of polychlorinated biphenyls impairs Leydig cellular steroidogenesis in F1 progeny rats Thangavelu SK.et al,Reprod Toxicol,2017
- EFFECT OF ADDING DRIED GINGER RHIZOMES TO DIETS ON SEMEN QUALITY AND FERTILITY RATE IN AGED LOCAL COCKS UNDER EGYPTIAN HOT Ezzat, W.et al,Egyptian Poultry Science Journal,2017
- Therapeutic effect of curcumin against nicotine-induced reproductive dysfunction in male rats Neveen A. Salem.et al,JIPBS,2017
- Effect of prenatal DINCH plasticizer exposure on rat offspring testicular function and metabolism Campioli E.et al,Sci Rep,2017
- Investigating the prenatal exposure of hydro-alcoholic extract of ginger on the function of Pituitary?Gonad axis in male mature offspring rats Nasim Zamani .et al,BASIC AND LABORATORY RESEARCH,2017
- The anorectic agent, lorcaserin, disturbs estrous cyclicity and produces endometrial hyperplasia without affecting ovarian population in female rats Abdel Latif RT.et al,Life Sci.,2017
- Reproductive toxicity after levetiracetam administration in male rats: Evidence for role of hormonal status and oxidative stress Merve Baysa.et al,PLOS ONE,2017
- Citalopram Induces Reproductive Toxicity in Male Rats Ilgin S.et al,Birth Defects Res,2017
- Protective Antioxidant Efficiency of Garlic against Lead-Induced Renal and Testicular Toxicity in Adult Male Rats Salem NA.et al,Heavy Metal Toxicity and Diseases,2016
- THERAPEUTICS FOR THE INDUCTION OF ENDOGENOUS STEROIDOGENESIS AND METHODS ASSOCIATED WITH THEIR IDENTIFICATION Papadopoulos V.et al, U.S. Patent ,2016
- Active immunization with GnRH-tandem-dimer peptide in young male rats reduces serum reproductive hormone concentrations, testicular development and spermatogenesis Han X F.et al,Asian journal of andrology,,2016
- Honey and Vitamin E Restore the Plasma Level of Gonadal Hormones and Improve the Fertilization Capacity in Noise-Stressed Rats Rajabzadeh A.et al,A. General Policy,2015
- Evaluation of offspring sex ratio, sex hormones and antioxidant enzymes following exposure to methyl tertiary butyl ether in adult male Sprague-Dawley rats. Khalili L.et al,EXCLI J.,2015
- The Effect of Intraventricular Administration of Zinc on Serum LH, FSH, Prolactin, and Testosterone in Male Rats Shahraki M R.et al,Zahedan Journal of Research in Medical Sciences,2015
- Surgical castration but not immuncastration is associated with reduced hypothalamic GnIH and GHRH/GH/IGF-I axis function in male rats Xingfa Han.et al,Theriogenology,2016
- Moxa smoke: Is it a beneficial or hazardous factor for infertility? A preclinical study on sperm parameters and sex hormones in male rats L Wang. et al,European Journal of Integrative Medicine,2015
- Increase in endogenous estradiol in the progeny of obese rats is associated with precocious puberty and altered follicular development in adulthood Ambrosetti V. et al,Endocrine,2016
- Honey and Vitamin E Restore the Plasma Level of Gonadal Hormones and Improve the Fertilization Capacity in Noise-Stressed Rats Rajabzadeh A.et al,A. General Policy,2015
- High-Cholesterol Diet Disrupts the Levels of Hormones Derived from Anterior Pituitary Basophilic Cells Yang J.et al,J Neuroendocrinol.,2016
- Disruptions in the hypothalamic-pituitary-gonadal axis in rat offspring following prenatal maternal exposure to lipopolysaccharide Izvolskaia MS.et al,Stress.,2016
- A Survey of the Relationship Between Noised Pollution, Honey and Vitamin E and Plasma Level of Blood Sexual Hormones in Noise-Exposed Rats Asghar Rajabzadeh et al,Jentashapir Journal of Health Research,2015
- Chronic Administration of High Doses of Nandrolone Decanoate on the Pituitary-Gonadal Axis in Male Rats Alsi枚 J. et al,Basic Clin Pharmacol Toxicol,2009
- Effect of honey on the reproductive system of male rat offspring exposed to prenatal restraint stress Haron MN. et al,Andrologia,2015
- Effects of low-dose cadmium exposure during gestation and lactation on development and reproduction in rats Luo X et al,Environ Sci Pollut Res Int,2015
- Introducing a rat model of prenatally androgen-induced polycystic ovary syndrome in adulthood Ramezani Tehrani F et al,Exp Physiol,2014
- A role for endocannabinoids in acute stress-induced suppression of the hypothalamic-pituitary-gonadal axis in male rats Karamikheirabad M et al,Clin Exp Reprod Med,2013
- Impact of 900 MHz electromagnetic field exposure on main male reproductive hormone levels: a Rattus norvegicus model Sepehrimanesh M et al,Int J Biometeorol,2013
- Intermittent fasting dietary restriction regimen negatively influences reproduction in young rats: a study of hypothalamo-hypophysial-gonadal axis Kumar S et al,PLoS One,2013
- Extended intervention time and evaluation of sperm suppression by dienogest plus testosterone undecanoate in male rat /,Contraception,2012
- Erectogenic and neurotrophic effects of icariin, a purified extract of horny goat weed (Epimedium spp.) in vitro and in vivo /,The Journal of Sexual Medicine,2010
- Clomiphene citrate potentiates the adverse effects of estrogen on rat testis and down-regulates the expression of steroidogenic enzyme genes Bharti S et al,Fertil Steril,2012
- Evaluation of effects of repeated sevoflurane exposure on rat testicular tissue and reproductive hormones Kaya Z et al,Inhal Toxicol.,2013
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靶点详情
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功能:Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
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基因功能参考文献:
- Orchestrated Egr1 and Pitx1 activities depending on specific central and peripheral regulatory inputs could be responsible for physiologically variable Lhb gene promoter activation in vivo. PMID: 25258388
- GnRH stimulates AMPK, and AMPK inhibition suppresses GnRH-stimulated LHbeta transcription. PMID: 23518923
- data suggest that the molecular decision for LHbeta subunit to enter the regulated secretory pathway is a pre-Golgi event controlled by the C-terminal heptapeptide. PMID: 23734233
- increased LH concentration induces production of incomplete LHR transcripts in hippocampal cells and decreases immature LHR at the protein level PMID: 23678542
- Data suggest that LH beta subunit expression (and FSH beta subunit expression) is regulated by DUSP1 expression (specifically in response to high-frequency GnRH pulses); this effect may occur in association with MAPK3/1 phosphorylation. PMID: 21228211
- Complex interactions between Ahr and Esr alter Prl and luteinizing hormone (LH) synthesis by direct actions in lactotropes and gonadotropes. PMID: 21187122
- LHbeta promoter activity is further regulated by the orphan nuclear receptors, chicken ovalbumin upstream promoter-transcription factors (COUP-TFI and COUP-TFII). PMID: 20797425
- data suggest that LHbeta G1502A polymorphism may be involved in the predisposition to infertility and minimal/mild endometriosis-associated infertility, although endometriosis might be only a coincidental finding along with infertility. PMID: 20430510
- PACAP may be released from the anterior pituitary cells in a concentration which would be able to influence LH release not only in vitro but under in vivo conditions as well. PMID: 12409218
- These results suggest that GnRH directly regulates LH secretion as well as LHbeta-subunit synthesis, and that LH2 cells are a useful model for the study of LH secretion induced by several secretagogues. PMID: 12940464
- androgens acting directly on the pituitary increase FSH-beta and decrease LH-beta transcription PMID: 14512439
- identify a second functional Pitx1 region spanning positions -73 to -52 in the LHbeta gene promoter PMID: 16087728
- JNK regulates both LHbeta and FSHbeta transcription in a differential manner. PMID: 17932215
- LHbeta, FSHbeta and the common alpha-subunit mRNA's were detected in intact follicles, theca cells, corpora lutea and in meiotically competent and incompetent oocytes. PMID: 18248883
- the carboxyl-terminal sequence in the lutropin beta subunit contributes to the sorting of lutropin to the regulated pathway PMID: 18292086
- In the 14-day-old rat pituitary, the majority of GHRH target cells are cells expressing LH beta mRNA alone or in combination with GH and/or POMC mRNA. PMID: 18363807
- Results demonstrate that degradation via the proteasome is vital to GnRH-stimulated LHbeta expression, and this occurs in part by allowing proper transcription factor associations with the LHbeta promoter. PMID: 19095772
- Lhb gene transcription in rat pituitary requires GnRH-induced early growth response 1 gene expression, which is mediated by MAPK8/9 and MAPK1/3. PMID: 19710510
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亚细胞定位:Secreted
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蛋白家族:Glycoprotein hormones subunit beta family
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数据库链接:
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