Rat Interleukin 10,IL-10 ELISA KIT

Datasheet
  • 中文名称:
    大鼠白介素10(IL-10)ELISA KIT
  • 货号:
    CSB-E04595r
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 基因名:
    Il10
  • 别名:
    Il10 ELISA Kit; Il-10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
  • 缩写:
    IL10
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围:
    3.12 pg/mL-200 pg/mL
  • 灵敏度:
    0.78 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %92
    Range %88-98
    1:2Average %96
    Range %91-101
    1:4Average %90
    Range %85-95
    1:8Average %93
    Range %90-96
  • 回收率:
    The recovery of rat IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-98
    EDTA plasma (n=4)9590-102
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.611 2.671 2.641 2.474
    1001.873 1.867 1.870 1.703
    501.206 1.234 1.220 1.053
    250.804 0.817 0.811 0.644
    12.50.515 0.527 0.521 0.354
    6.250.387 0.399 0.393 0.226
    3.120.314 0.327 0.321 0.154
    00.155 0.179 0.167  
  • 货期:
    3-5 working days

引用文献

问答及客户评论

 客户评论
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Culture supernatants
    Sample species: Rat
    Review: The levels of cytokines in the different groups (n = 9). The production of IL-10 in supernatant of cultured PMNs was detected by ELISA. PMNs were cultured with NPS-2143 (100 nM), cinacalcet (100 nM), and PDTC (100 nM) for 12 h after stimulated by fMLP (10 nM) for 2 min. #P < 0 05 versus control group, ∗P < 0 05 versus fMLP group, and △P < 0 01 versus fMLP + CIN group.
    PMID: 29081886
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Culture supernatants
    Sample species: Rat
    Review: HO‐1 overexpression increases the production of paracrine factors from MSCs under PC stimulation. PCs were applied to all types of stem cells for 7 days, and then the levels of IL-10 (g) in culture supernatants were analyzed by enzyme-linked immunosorbent assays. All data are expressed as mean ± SEM (n = 6). *p < 0.05 versus MSC + PBS and MSC‐HO‐1 + PBS groups, #p < 0.05 versus MSC + PC group, and Δp < 0.05 versus MSC-HO-1 + PC group.
    PMID: 30362554
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Colorectal tissues
    Sample species: Rat
    Review: The anti-inflammatory cytokines IL-10 is decreased to a low level after TNBS exposure, whereas treatment with middle- or high-dose of BD-SNEDDS, BD-suspension, or AZA notably restored these anti-inflammatory cytokines. Data are expressed as mean±SD and analyzed by ANOVA followed by Dunnett’s test. ##P,0.01 vs intact group, **P,0.01 and *P,0.05 vs the vehicle group, P,0.05 and P,0.01 vs BD-suspension group.
    PMID: 30319255
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Tissues
    Sample species: Rat
    Review: IL-10 levels were obtained in treated and untreated groups (a) and comparison to the highest level obtained for each cytokine in periodontitis group (positive control).
    PMID: 30194012
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Culture supernatants
    Sample species: Rat
    Review: Compared with the vector-NC group, decreased  IL-10 level were found in the P2X7-vector group (p < 0.05).
    PMID: 30892077
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Plamsa
    Sample species: Rat
    Review: Effect of KJL on IL-1 in plasma. Data are presented as the mean ± SD. ##P < 0.01, #P < 0.05 versus the control group; **P < 0.01, *P < 0.05 versus the TNBS group. (n = 8–12).
    PMID: 28750820
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Liver homogenate tissue
    Sample species: Rat
    Review: Effect of PTX on BDL-induced changes in liver IL-10, fourteen days post-operation, paclitaxel (PTX) (0.3 mg/kg/ip) was administered three times a week for 2 weeks. Hepatic IL-10 level were measured thereafter. Data are expressed as mean ± SD (n=6).
    PMID: 30243650
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Culture supernatants
    Sample species: Rat
    Review: Nrf2 knockdown reversed the anti-inflammatory effects of SalC. Nrf2 siRNA reversed SalC-induced IL‑10 downregulation. All data are presented as mean ± SD of four independent experiments. ##P < 0.01 vs control group, ⁎⁎P < 0.01 vs LPS group, scale bar = 20 μm.
    PMID: 30142530
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Jejunal mucosa tissues
    Sample species: Rat
    Review: Effect of ethanolamine on cytokines of weaned rats. The concentrations of cytokines in the jejunal mucosa were not different among groups.
    PMID: 29789027
  • Western blot review for CD146 Monoclonal Antibody
    Sample type: Brain tissues
    Sample species: Rat
    Review: Concentrations of the IL10 and their receptors were significantly lower in the serum than in the central nervous system in both SH and WKY rats (p < 0.001).
    PMID: 30539780

靶点详情

  • 功能:
    Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 基因功能参考文献:
    1. Low IL10 expression is associated with liver fibrosis. PMID: 30243650
    2. A modified pectic polysaccharide from turmeric (Curcuma longa) with antiulcer effects via anti-secretary, mucoprotective and IL-10 mediated anti-inflammatory mechanisms. PMID: 29924982
    3. IL10 suppressed inflammation, facilitated retinal pigment epithelium cell apoptosis and inhibited cell proliferation and migration through the regulation of VEGF expression in rhegmatogenous retinal detachment. PMID: 29568872
    4. ghrelin with high doses (80 and 160 mug/kg) significantly reduced pain intensity in both the early and the late phases of pain. The serum levels of cytokines, IL-10, and TGF-beta1 showed a significant elevation with ghrelin at the dose of 160 mug/kg. PMID: 27703278
    5. IL-10 not only attenuates neuronal apoptosis, but also promotes neurite outgrowth and synapse formation via the JAK1/STAT3 signaling pathway. PMID: 27456198
    6. both the AV and the LV vector systems were able to transfect limbal graft tissue ex vivo with biologically active IL-10, leading to delayed rejection compared to the controls. PMID: 28925732
    7. Addition of 320 (48) and 640 (96) mg/L (mg/kg BW) boron markedly reduced the serum IgG concentrations; splenic IL-2 and IL-10 expression; the number of CD3(+), CD4(+) and PCNA(+) cells; and increased the number of splenic CD8(+) and caspase-3(+) cells and promoted caspase-3 expression in CD3(+) cells. PMID: 28092075
    8. The expression levels of IL-10, NF-kappaB and MMP-3 in the intervertebral disc tissue samples were higher in test animals than in controls at one-month, three-months and six-months after surgically induced cervical spondylosis. Cervical degeneration may be accompanied by changes in IL-10. PMID: 29197741
    9. Comparative Modulation of Levels of Oxidative Stress in the Liver of Anti-Tuberculosis Drug Treated Wistar Rats by Vitamin B12, Beta-Carotene, and Spirulina fusiformis: Role of NF-kappaB, iNOS, IL-6, and IL-10.( PMID: 28387444
    10. the effects of dapagliflozin on STAT3 activity and myocardial IL-10 levels can be reversed by 3-morpholinosydnonimine, a peroxynitrite generator. Taken together, these observations provide a novel mechanism of SGLT2 inhibitors-mediated M2 polarization through a RONS-dependent STAT3-mediated pathway and selective SGLT2 inhibitors are more effective in attenuating myofibroblast infiltration during postinfarction remodeling PMID: 28132924
    11. TNF-alpha, IL-6 and IL-10 expressions, responsible for disparity in action of curcumin against cisplatin-induced nephrotoxicity in rats. PMID: 28258441
    12. this study shows that transduction of interleukin-10 through renal artery attenuates vascular neointimal proliferation and infiltration of immune cells in rat renal allograft PMID: 27317647
    13. this study shows that that N-Acetyl-l-cysteine acts as an antipyretic via IL-10 stimulation PMID: 27363620
    14. Transplantation of mesenchymal stem cells overexpressing IL10 preserves myocardial viability and left ventricular rfunction in rats with myocardial infarction. PMID: 28322445
    15. The bactericidal activity of caspase-6-/- macrophages was impaired compared to wild type cells. Caspase-6-/- mice showed higher expression of the IL-1b gene, known to be detrimental in murine melioidosis. Expression of the IL-10 gene was also increased in caspase-6-/- mice as early as 6 hours after infection. Treatment with exogenous IL-10 rendered mice more susceptible against B. pseudomallei challenge PMID: 28686630
    16. use of rebamipide retards CCl4-induced hepatic fibrosis, which is associated with significant increases in liver levels of IL-10 PMID: 26849241
    17. Data suggest that vitamin A, when used as an unnecessary dietary supplement, decreases skeletal muscle expression of anti-inflammatory mediators interleukin-10 and heat shock protein 70, important factors for positive exercise adaptations and tissue damage prevention. PMID: 28368329
    18. Exercise combined with ultrasound attenuates neuropathic pain in rats and is associated with downregulation of IL-6 and TNF-alpha, and with upregulation of IL-10. PMID: 28319508
    19. Results uncover a novel neuroimmune modulation of GABAergic neurotransmission as well as a novel role for IL-10 in response to acute ethanol intake. Early increase in IL-10 levels may contribute to the complex changes in neuroimmune signaling that occur after acute exposure to ethanol. Further, IL-10 has an inhibitory effect on GABAergic transmission, possibly leading to inhibition of sleep PMID: 27016017
    20. Nonsteroidal Anti-Inflammatory Drugs data provide opportunities to investigate such associations and specifically imply that potential interrelationships among OTC NSAID use, IL-10 genotype, and IL-10 cytokine levels deserve further study PMID: 28135825
    21. The expression of IL-10 and TGF-beta in the CD25 siRNA group increased, while IFN-gamma, IL-1beta, and TNF-alpha expression decreased, as well as no significant changes in Foxp3 expression were observed at day 14 post-operation. PMID: 27567557
    22. IL10 has a role in preventing -induced cell death in rat hippocampal cultures PMID: 28063948
    23. IL-10 592 polymorphism was associated with diabetic retinopathy susceptibility, suggesting that the gene polymorphism might be a risk factor for diabetic retinopathy PMID: 26492380
    24. Mesenchymal stromal cells inhibit Human antigen R (HuR) trascription, an enhancer of RENmRNA stability by IL10 release PMID: 26866372
    25. Mesenchymal stem cells decrease apoptosis and improve learning-memory function in hypoxic-ischemic brain damage rats by suppressing the TLR2/NFkappaB signaling pathway via a feedback mechanism that reduces IL-10 release. PMID: 26475712
    26. Green tea intake reduces expression of the pro-inflammatory cytokine TNF-alpha and the osteoclastogenic mediator RANKL to normal levels while increasing expression of the anti-inflammatory cytokine IL-10. PMID: 26270535
    27. IL-10 released from activated glial cells in the VLO can potentially attenuate allodynia while IL-1beta released from activated glial cells in the VLO can potentially maintain or facilitate allodynia PMID: 25617163
    28. The objectives of these studies were to investigate the responses of IL-10 receptors (IL-10Rs) and IL-10 signalling proteins (IL-10SPs) in hyperoxic foetal alveolar type II cells. PMID: 26059905
    29. Leydig cell-conditioned media dose-dependently stimulated expression of transcription factor Foxp3 and secretion of IL-10 in splenic CD4+ T cells, an effect abolished by addition of the anti-androgen flutamide. PMID: 25598450
    30. Exercise increased the amount of IL-1beta and IL-10 positive chondrocytes PMID: 25702530
    31. Study shows that, in addition to inducing persistent memory deficits, maternal deprivation increased the levels of the anti-inflammatory cytokine, IL-10, and of the pro-inflammatory cytokine, TNF-alpha, and decreased the levels of BDNF in the adulthood PMID: 25182413
    32. atropine in malathion intoxicated rats had no effect on suppression of the synthesis of proinflammatory cytokines TNF, IL-1g and IL-6 as well as the content of anti-inflammatory cytokines IL-10 and IL-13. PMID: 26591203
    33. Short-term but not long-term hypoglycaemia enhances plasma levels and hepatic expression of HSP72 in insulin-treated rats: an effect associated with increased IL-6 levels but not with IL-10 or TNF-alpha. PMID: 25096025
    34. the preserved ability of macrophages from aged AO rats to synthesize not only proinflammatory TNF-alpha, but also immunoregulatory IL-10 cytokine most likely contributes to their longer average life compared with DA rats PMID: 25009084
    35. The dysfunction of dendritic cells and the increase of IL-10-producing Breg and Foxp3(+) Treg might play important roles in hepatocellular carcinoma progression PMID: 25066760
    36. SOCS-3 was involved in cerebral vasospasm after subarachnoid hemorrhage via an IL-6- and IL-10-related mechanism. IL-10 levels increased during the first 6 h after SAH, after which this increase tapered off. PMID: 24463741
    37. results suggest that IL-10/HMOX1 signaling is involved in modulation of cochlear inflammation through inhibition of MCP-1/CCL2 regulation in SLFs PMID: 25780042
    38. The neuronal apoptosis rate was positively correlated with the levels of TNF-alpha and IL-6, and negatively correlated with IL-10 and GDNF. PMID: 24706318
    39. cells from long-lived rats exhibited a dramatic increase in IL-10 mRNA expression followed by diminished TNF-alpha and IL-6 mRNA expression, and comparable expression of IL-1beta mRNA relative to adult rats. PMID: 25081109
    40. Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10. PMID: 25329858
    41. Regulation of IL-10 by chondroitinase ABC promotes a distinct immune response following spinal cord injury PMID: 25471580
    42. We found cardiac and histological changes compatible with the inflammatory process triggered by acute pancreatitis with the promotion of local myocardial cytokine production. PMID: 24865534
    43. study provides novel evidence that IL-10 plays an up-regulatory role in the anti-hypertensive activity of CCL5 in SHR VSMCs PMID: 24656930
    44. The high expression in IL-1beta and IL-10 levels in small bowel and colon might explain the accelerated healing process in these wounds in comparison to skin and muscle tissues. PMID: 24114903
    45. Daily treatment with anti-IL10 antibody significantly increased paw edema. PMID: 23765634
    46. Prevention of acute liver allograft rejection by IL-10-engineered mesenchymal stem cells. PMID: 24527865
    47. Gemfibrozil plus metformin decrease MMP-9, increase IL-10 and adiponectin acting. PMID: 23111552
    48. Pro-inflammatory cytokines from area postrema and organum vasculosum laminae terminalis are modulated by IL-10 antibodies. PMID: 23388469
    49. GSK-3beta modulates inflammatory IL-10 levels released from cortical glia. PMID: 22796213
    50. These data in cardiomyocytes suggest that IL-10 induced anti-apoptotic signaling involves upregulation of TLR4 through MyD88 activation. PMID: 23141143

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  • 亚细胞定位:
    Secreted
  • 蛋白家族:
    IL-10 family
  • 数据库链接:

    KEGG: rno:25325

    STRING: 10116.ENSRNOP00000006246

    UniGene: Rn.9868