Rat Insulin-like growth factor binding protein 2,IGFBP-2 ELISA kit

Datasheet
  • 中文名称:
    大鼠胰岛素样生长因子结合蛋白2(IGFBP-2)ELISA Kit
  • 货号:
    CSB-E07351r
  • 规格:
    96T
  • 价格:
    3200
  • 其他:

产品详情

  • 别名:
    Igfbp2 ELISA Kit; Igfbp-2Insulin-like growth factor-binding protein 2 ELISA Kit; IBP-2 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP-2 ELISA Kit; BRL-BP ELISA Kit
  • 缩写:
    IGFBP2
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.625 ng/mL-40 ng/mL
  • 灵敏度:
    0.156 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:20 Average % 107
    Range % 95-111
    1:40 Average % 104
    Range % 95-108
    1:80 Average % 90
    Range % 83-94
    1:160 Average % 97
    Range % 87-103
  • 回收率:
    The recovery of rat IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 90 82-94
    EDTA plasma (n=4) 101 90-105
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.631 2.568 2.600 2.439
    20 2.014 1.964 1.989 1.828
    10 1.126 1.083 1.105 0.944
    5 0.589 0.567 0.578 0.417
    2.5 0.347 0.356 0.352 0.191
    1.25 0.266 0.277 0.272 0.111
    0.625 0.219 0.212 0.216 0.055
    0 0.159 0.163 0.161  
  • 货期:
    3-5 working days

靶点详情

  • 功能:
    Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 基因功能参考文献:
    1. Insulin-like growth factor 2 (IGF2) is an important regulator of fetal growth. PMID: 24352370
    2. Abnormal expression of IGFBP-2 correlates with hyperoxia-induced lung injury in neonatal rats. PMID: 18549709
    3. Depolarization induces insulin-like growth factor binding protein-2 expression in vivo via NMDA receptor stimulation. PMID: 11914028
    4. results show that liver growth and cell proliferation in association with insulin-like growth factor-I and insulin-like growth factor binding proteins 1 and 2 levels are affected in utero by fetal undernutrition PMID: 12902319
    5. In proliferating astrocytes, IGFBP-2 may help target insulin-like growth factors (IGFs) to IGF-1 receptors, and IGFBP-2 proteases may play a role in enhancing the availability of IGFs. PMID: 15248291
    6. cathepsin L created a 14-kDa carboxyl IGFBP-2 fragment that corresponded to the sole antigen detected in IEC-18 cell lysates as well as a 16.5-kDa fragment found in the media PMID: 15705658
    7. retinoic acid modulates the secreted IGFBP-2 and -3 during O(2) exposure and inhibits the increase in collagen that occurs during lung injury PMID: 16055936
    8. Growth inhibition, which was supported by the strong induction of p21CIP1/WAF1, was correlated with increased secretion of IGFBP-2 and, unexpectedly, with its increased localization in the nucleus and particularly in the cytoplasm. PMID: 16131350
    9. IGFBP-2 plays a role in the lamellar bone formation process. PMID: 17308996
    10. In conclusion, MMP-9-induced IGFBP2 proteolysis may be regarded as an important post-translational event involved in astrocytoma aggressiveness. These new findings support drug targeting of MMP-9 as an interesting approach in the treatment of PMID: 18563800
    11. A brain region- and cell type-specific responses to estrogen in rat brain, suggesting that Igf signaling may mediate the estrogen function in the hippocampus. PMID: 18946176

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  • 亚细胞定位:
    Secreted
  • 组织特异性:
    In adults, expressed in brain, testes, ovaries, and kidney. Expression in the adult liver is barely detectable.
  • 数据库链接:

    KEGG: rno:25662

    STRING: 10116.ENSRNOP00000023068

    UniGene: Rn.6813