Rat Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Datasheet
  • 中文名称:
    大鼠Ⅲ型胶原(Col Ⅲ)ELISA Kit
  • 货号:
    CSB-E07924r
  • 规格:
    96T
  • 价格:
    3900
  • 其他:

产品详情

  • 别名:
    Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
  • 缩写:
    Col Ⅲ
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.156 ng/mL-10 ng/mL
  • 灵敏度:
    0.039 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-96  
    1:2 Average % 98  
    Range % 92-104  
    1:4 Average % 95  
    Range % 92-98  
    1:8 Average % 86  
    Range % 80-94  
  • 回收率:
    The recovery of rat Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 90 85-95  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    10 2.585 2.532 2.559 2.415  
    5 1.795 1.719 1.757 1.613  
    2.5 1.083 1.021 1.052 0.908  
    1.25 0.657 0.705 0.681 0.537  
    0.625 0.418 0.450 0.434 0.290  
    0.312 0.299 0.314 0.307 0.163  
    0.156 0.242 0.229 0.236 0.092  
    0 0.146 0.141 0.144    
  • 货期:
    3-5 working days