Rat Caspase-1(CASP1) ELISA kit

Datasheet
  • 中文名称:
    大鼠半胱氨酸天冬氨酸蛋白酶1(CASP1) ELISA kit
  • 货号:
    CSB-EL004543RA
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 别名:
    Casp1 ELISA kit; Il1bc ELISA kit; Il1bceCaspase-1 ELISA kit; CASP-1 ELISA kit; EC 3.4.22.36 ELISA kit; Interleukin-1 beta convertase ELISA kit; IL-1BC ELISA kit; Interleukin-1 beta-converting enzyme ELISA kit; ICE ELISA kit; IL-1 beta-converting enzyme ELISA kit; p45) [Cleaved into: Caspase-1 subunit p20; Caspase-1 subunit p10] ELISA kit
  • 缩写:
    CASP1
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, cell lysates, cell culture supernates
  • 检测范围:
    6.25 pg/mL-400 pg/mL
  • 灵敏度:
    1.56 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cell Biology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
      Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 88-93    
    1:2 Average % 95    
    Range % 90-102    
    1:4 Average % 97    
    Range % 94-101    
    1:8 Average % 94    
    Range % 91-98    
  • 回收率:
    The recovery of rat CASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 90 86-94    
    EDTA plasma (n=4) 99 93-105    
                   
                   
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    \"\"
    pg/ml OD1 OD2 Average Corrected    
    400 2.189 2.321 2.255 2.047    
    200 1.729 1.754 1.742 1.534    
    100 1.151 1.165 1.158 0.950    
    50 0.755 0.748 0.752 0.544    
    25 0.456 0.468 0.462 0.254    
    12.5 0.317 0.325 0.321 0.113    
    6.25 0.277 0.290 0.284 0.076    
    0 0.201 0.215 0.208      
  • 货期:
    5-7 working days

靶点详情

  • 功能:
    Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive.
  • 亚细胞定位:
    Cytoplasm
  • 蛋白家族:
    Peptidase C14A family
  • 数据库链接: