Rat 6-keto-PGF1α ELISA Kit

Datasheet
  • 中文名称:
    大鼠6-酮前列腺素F1α(6-keto-PGF1α)ELISA Kit
  • 货号:
    CSB-E14411r
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 别名:
    6-keto-PGF1α,6-keto-PGF1 alpha
  • 缩写:
    6-keto-PGF1α
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates, cell culture supernates
  • 检测范围:
    31.25 pg/mL-2000 pg/mL
  • 灵敏度:
    7.81 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat 6-keto-PGF1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %85
    Range %80-90
    1:2Average %88
    Range %82-95
    1:4Average %90
    Range %85-95
    1:8Average %92
    Range %84-97
  • 回收率:
    The recovery of rat 6-keto-PGF1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-100
    EDTA plasma (n=4)9893-103
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    20002.151 2.105 2.128 2.036
    10001.744 1.659 1.702 1.610
    5001.328 1.294 1.311 1.219
    2500.972 0.896 0.934 0.842
    1250.627 0.602 0.615 0.523
    62.50.313 0.289 0.301 0.209
    31.250.196 0.191 0.194 0.102
    00.095 0.088 0.092  
  • 货期:
    3-5 working days