Rat 5-Hydroxytryptamine,5HT ELISA Kit

Datasheet
  • 中文名称:
    大鼠5羟色胺(5-HT)ELISA Kit
  • 货号:
    CSB-E08364r
  • 规格:
    96T
  • 价格:
    3900
  • 其他:

产品详情

  • 缩写:
    5HT
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, cell lysates, tissue homogenates
  • 检测范围:
    0.8 ng/mL-160 ng/mL
  • 灵敏度:
    0.4 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-98  
    1:2 Average % 95  
    Range % 92-99  
    1:4 Average % 97  
    Range % 89-103  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率:
    The recovery of rat 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 96 92-103  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    160 0.069 0.074 0.072    
    40 0.181 0.195 0.188    
    12.8 0.426 0.439 0.433    
    3.2 0.802 0.855 0.829    
    0.8 1.155 1.231 1.193    
    0 2.158 2.227 2.193    
  • 货期:
    3-5 working days

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