Rat 5-Hydroxytryptamine,5HT ELISA Kit

Datasheet
  • 中文名称:
    大鼠5羟色胺(5-HT)ELISA Kit
  • 货号:
    CSB-E08364r
  • 规格:
    96T
  • 价格:
    3900
  • 其他:

产品详情

  • 产品描述:

    The Rat 5-Hydroxytryptamine (5HT) ELISA kit is used to detect and quantify the concentrations of INS in serum, plasma, cell lysates, and tissue homogenates. This kit exclusively recognizes rat 5HT proteins. This ELISA kit has been confirmed to have high sensitivity, strong specificity, premium precision, high recovery, good linearity, and lot-to-lot consistency. See the instructions for more details.

    Standards or samples are added to the appropriate microtiter plate wells pre-coated with goat-anti-rabbit 5HT antibody. HRP-conjugated 5HT and anti-rat 5HT antibody are pipped into the wells, where 5HT in the sample or standard competes with the HRP labeled 5HT for limited binding sites on the anti-rat 5HT antibody. These Ag-Ab complexes are captured by the antibody immobilized on the plate. The solution color turns blue after the addition of substrate A and B. The color reaction is immediately terminated after the addition of the stop solution. And the color changes from blue to yellow. The color develops in opposition to the amount of 5HT in the sample, and the intensity of the color is measured by a Microplate reader at 450 nm.

    5-HT, also called serotonin, is a monoamine neurotransmitter that plays a critical role in the central nervous system (CNS). 5-HT effects are mediated through its interaction with 5-HT receptors (5-HTRs). 5-HT regulates mood stability, pain tolerance, and sleep patterns. It is also involved in the modulation of individual behaviors that organize social group dynamics.

  • 缩写:
    5HT
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, cell lysates, tissue homogenates
  • 检测范围:
    0.8 ng/mL-160 ng/mL
  • 灵敏度:
    0.4 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Neuroscience
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat 5-HT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-98  
    1:2 Average % 95  
    Range % 92-99  
    1:4 Average % 97  
    Range % 89-103  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率:
    The recovery of rat 5-HT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 96 92-103  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    160 0.069 0.074 0.072    
    40 0.181 0.195 0.188    
    12.8 0.426 0.439 0.433    
    3.2 0.802 0.855 0.829    
    0.8 1.155 1.231 1.193    
    0 2.158 2.227 2.193    
  • 本试剂盒所含材料:
    • A 96-well Assay plate -- The 96-well plate has been pre-coated with goat-anti-rabbit 5HT antibody.
    • Standard 6 x 0.5 ml -- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
    • Anti-rat 5HT Antibody 1 x 6 ml -- Act as the detection antibody.
    • HRP-conjugated 5HT 1 x 6 ml -- Bind to the 5HT antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
    • Wash Buffer (20 x concentrate) 1 x 15 ml -- Wash away unbound or free substances.
    • Substrate A 1 x 7 ml -- Mix with substrate B, and the TMB is catalyzed by HRP to develop color.
    • Substrate B 1 x 7 ml -- Mix with substrate A, and the TMB is catalyzed by HRP to develop color.
    • Stop Solution 1 x 7 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual

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  • 本试剂盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

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  • 货期:
    3-5 working days

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