Rat 3-nitrotyrosine (3-NT) ELISA Kit

Datasheet
  • 中文名称:
    大鼠3-硝基酪氨酸ELISA Kit
  • 货号:
    CSB-EQ027348RA
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 缩写:
    3-NT
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma
  • 检测范围:
    0.156 ng/mL-10 ng/mL
  • 灵敏度:
    0.039 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat 3-NT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %90
    Range %85-96
    1:2Average %87
    Range %82-94
    1:4Average %90
    Range %85-95
    1:8Average %99
    Range %93-105
  • 回收率:
    The recovery of rat 3-NT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8580-91
    EDTA plasma (n=4)9285-98
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.289 2.107 2.198 2.087
    51.945 1.852 1.899 1.788
    2.51.553 1.542 1.548 1.437
    1.251.236 1.213 1.225 1.114
    0.6250.814 0.774 0.794 0.683
    0.3120.572 0.527 0.550 0.439
    0.1560.291 0.269 0.280 0.169
    00.111 0.110 0.111  
  • 货期:
    7-14 working days

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