Mouse procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Mouse procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

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中文名称：

小鼠Ⅰ型前胶原N端前肽(PⅠNP)ELISA Kit
货号：

CSB-E12775m
规格：

96T
价格：

3600
其他：

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别名：

procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
缩写：

PⅠNP
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.7 pg/mL-300 pg/mL
灵敏度：

1.17 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PINP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-98
1:200	Average %	95
	Range %	91-98
1:400	Average %	91
	Range %	85-96
1:800	Average %	94
	Range %	88-102

回收率：

The recovery of mouse PINP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-99
EDTA plasma (n=4)	89	82-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
300	2.413	2.313	2.363	2.261
150	1.598	1.498	1.548	1.446
75	0.940	0.890	0.915	0.813
37.5	0.489	0.479	0.484	0.382
18.75	0.316	0.306	0.311	0.209
9.4	0.207	0.205	0.206	0.104
4.7	0.145	0.142	0.144	0.042
0	0.103	0.101	0.102

货期：

3-5 working days

Conditional knockout of the PDK‐1 gene in osteoblasts affects osteoblast differentiation and bone formation Y Bai,Journal of Cellular Physiology,2021
Acanthopanax senticosus aqueous extract ameliorates ovariectomy-induced bone loss in middle-aged mice by inhibiting the receptor activator of nuclear factor-κB ligand-induced osteoclastogenesis H Xu,Food & Function,2020
Hydrogen sulfide protects against particle┸\induced inflammatory response and osteolysis via SIRT1 pathway in prosthesis loosening Liu L,FASEB,2020
The emerging role of IMD 0354 on bone homeostasis by suppressing osteoclastogenesis and bone resorption, but without affecting bone formation Chen W, et al,Cell Death and Disease,2019
A novel transgenic murine model with persistently brittle bones simulating osteogenesis imperfecta type I Yi Liu, et al,Bone,2019
Malt1 deficient mice develop osteoporosis independent of osteoclast-intrinsic effects of Malt1 deficiency Monajemi M, et al,Journal of Leukocyte Biology,2019
A novel anti-osteoporotic agent that protects against postmenopausal bone loss by regulating bone formation and bone resorption Yu-te Yang .et al,Life Sciences,2018
A Novel Diterpenoid Suppresses Osteoclastogenesis and Promotes Osteogenesis by Inhibiting Ifrd1-and IκBα-Mediated p65 Nuclear Translocation Xie, Zi\'ang.et al,Journal of Bone and Mineral Research ,2017
Effect of alendronate on post-traumatic osteoarthritis induced by anterior cruciate ligament rupture in mice Khorasani MS.et al,Arthritis Res Ther.,2015
Altered bone development in a mouse model of peripheral sensory nerve inactivation. Heffner MA et al,J Musculoskelet Neuronal Interact,2014

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.