Mouse procollagen Ⅰ N-terminal peptide,PⅠNP ELISA Kit

Datasheet
  • 中文名称:
    小鼠Ⅰ型前胶原N端前肽(PⅠNP)ELISA Kit
  • 货号:
    CSB-E12775m
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 别名:
    procollagen 1 N-terminal peptide,P1NP,_x000D_ procollagen I N-terminal peptide,PINP
  • 缩写:
    PⅠNP
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    4.7 pg/mL-300 pg/mL
  • 灵敏度:
    1.17 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PINP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 92  
    Range % 87-98  
    1:200 Average % 95  
    Range % 91-98  
    1:400 Average % 91  
    Range % 85-96  
    1:800 Average % 94  
    Range % 88-102  
  • 回收率:
    The recovery of mouse PINP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-99  
    EDTA plasma (n=4) 89 82-94  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    300 2.413 2.313 2.363 2.261  
    150 1.598 1.498 1.548 1.446  
    75 0.940 0.890 0.915 0.813  
    37.5 0.489 0.479 0.484 0.382  
    18.75 0.316 0.306 0.311 0.209  
    9.4 0.207 0.205 0.206 0.104  
    4.7 0.145 0.142 0.144 0.042  
    0 0.103 0.101 0.102    
  • 货期:
    3-5 working days

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