Mouse Transforming Growth factor β1,TGF-β1 ELISA kit

Datasheet
  • 中文名称:
    小鼠转化生长因子β1(TGF-β1)ELISA Kit
  • 货号:
    CSB-E04726m
  • 规格:
    96T
  • 价格:
    3800
  • 其他:

产品详情

  • 产品描述:

    The Mouse Transforming Growth factor β1 (TGF-β1) ELISA kit is a solid phase immunoassay specially designed to quatitatively measure mouse TGF-β1 in serum, plasma, or cell culture supernates. It is based on the Sandwich-ELISA mechainsm. TGF-β1 in the sample is bound to the capture antibody immobilized on the 96-well strip plate and then sandwiched with the biotinylated TGF-β1 antibody. After the addition of HRP-avidin and TMB substrate, the solution in the wells turns blue. The color reaction is stopped by adding the stop solution into the wells, and the color changes from blue to yellow. The color intensity is positively proportional to the TGF-β1 bound in the initial step. The TGF-β1 concentration can be calculated according to the standard curve. This kit is tested with high sensitivity, strong specificity, good linearity, precision less than 10%, recovery with 87%-100%, and lot-to-lot cosistency. More validation information is described in the product instructions.

    TGF-β1 is one of TGF-β isoforms. TGF-β1 is the most common in the immune system, in which it acts as a crucial pleiotropic cytokine with potent immunoregulatory properties. It has a multitude of functions, including both promoting the formation and inhibiting the breakdown of connective tissue. It also inhibits epithelial cell proliferation. Additionally, TGF-β1 plays an important role in both normal and abnormal wound healing. TGF-β1/TGF-βRII signaling mediates a wide range of pathways, including the Erk/MAPK pathway and the PI3K/Akt pathway, finally promoting cell proliferation, differentiation, and migration.

  • 别名:
    Tgfb1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
  • 缩写:
    TGFB1
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, cell culture supernates
  • 检测范围:
    0.78 ng/mL-50 ng/mL
  • 灵敏度:
    0.2 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 99  
    Range % 94-104  
    1:2 Average % 89  
    Range % 83-97  
    1:4 Average % 92  
    Range % 87-97  
    1:8 Average % 92  
    Range % 87-96  
  • 回收率:
    The recovery of mouse TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-99  
    EDTA plasma (n=4) 96 90-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    50 2.654 2.636 2.645 2.515  
    25 2.318 2.455 2.387 2.257  
    12.5 1.756 1.886 1.821 1.691  
    6.25 0.997 1.001 0.999 0.869  
    3.12 0.542 0.512 0.527 0.397  
    1.56 0.301 0.309 0.305 0.175  
    0.78 0.228 0.238 0.233 0.103  
    0 0.129 0.131 0.130    
  • 本试剂盒所含材料:
    • A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse TGF-β1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
    • Two vials lyophilized standard --- Dilute a bottle of standard at dilution series, read the OD values, and then draw a standard curve.
    • Biotin-labeled TGF-β1 antibody (100 x concentrate) 1 x 120 μl ---Act as the detection antibody.
    • HRP-avidin (100 x concentrate) 1 x 120 μl --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
    • Biotin-antibody Diluent 1 x 15 ml ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
    • HRP-avidin Diluent 1 x 15 ml---Dilute the high concentration HRP-avidin solution to an appropriate solution.
    • Sample Diluent 1 x 50 ml---Dilute the sample to an appropriate concentration.
    • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away unbound or free substances.
    • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
    • Stop Solution 1 x 10 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
    • An instruction manual

    显示更多

    收起更多

  • 本试剂盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution

    显示更多

    收起更多

  • 货期:
    3-5 working days

引用文献

靶点详情

  • 功能:
    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts
  • 基因功能参考文献:
    1. this paper shows that epithelial-derived TGF-beta1 acts as a pro-viral factor in the lung during influenza A infection PMID: 29067998
    2. Study points toward elevated levels of active TGF-beta as inducers and promoters of ectopic bone formation, and suggest that TGF-beta might be a therapeutic target in heterotopic ossification. PMID: 29416028
    3. The comparison of transforming growth factor beta family (TGFbeta) expression showed significantly higher levels of Tgfbeta3 transcript between nude and Balb/c mice but no differences were detected for Tgfbeta1. Nude DFs were specifically sensitive to the presence of the pro-regenerative TGFbeta3 isoform, showing increased collagen I deposition and alpha smooth muscle actin expression. PMID: 29637306
    4. Results indicate that the miR-23a cluster regulates osteocyte differentiation by modulating the TGF-beta signalling pathway. PMID: 28397831
    5. Following Schistosoma exposure, TSP-1 levels in the lung increase, via recruitment of circulating monocytes, while TSP-1 inhibition or knockout bone marrow prevents TGF-beta activation and protects against pulmonary hypertension development. PMID: 28555642
    6. TGF-beta/Smad proteins signaling affects radiation response and prolongs survival by regulating DNA repair genes in malignant glioma. PMID: 30230914
    7. The results indicate that EGFR and its activation are critical for YAP-mediated suppression of TGF-beta1-induced apoptosis. This study provides a new understanding of the regulatory mechanism underlying the determination of cell fate in response to TGF-beta1-mediated simultaneous apoptosis and epithelial mesenchymal transformation. PMID: 28425446
    8. transforming growth factor beta (TGFbeta) signaling was upregulated in HSCs from bone marrow of mice with MLL-AF9-induced acute myeloid leukemia (AML) because of excessive production of TGFbeta1, especially from megakaryocytes, and overactivation of latent TGFbeta1 protein. PMID: 29307605
    9. TGF-beta release from platelets is necessary for podoplanin-mediated tumor invasion and metastasis in lung cancer. PMID: 28176852
    10. Data suggest partial or complete transforming growth factor beta 1 (TGFBI) knockdown as a potential therapy against TGFBI-linked corneal dystrophies. PMID: 29117645
    11. De novo formation of the biliary system by TGFbeta-mediated hepatocyte transdifferentiation PMID: 29720662
    12. CXCL9 may promote prostate cancer progression via inhibition of cytokines from T cells. PMID: 29901197
    13. EGCG attenuated airway inflammation in asthmatic mice, decreased the percentage of Th17 cells and increased the percentage of Treg cells. The antiinflammatory effect of EGCG is achieved via the TGFbeta1 signaling pathway. PMID: 29916550
    14. fibrosis induced by Ang II may be alleviated by AKAP12 expression through inactivation of the TGF-beta1 pathway. PMID: 29501491
    15. RUNX1 promoted TGF-beta-induced partial EMT by increasing transcription of the PI3K subunit p110delta, which mediated Akt activation. PMID: 29759484
    16. Transforming growth factor-beta1 induces cerebrovascular dysfunction and astrogliosis through angiotensin II type 1 receptor-mediated signaling pathways PMID: 29505736
    17. TET2 plays an important role in the pathogenesis of diabetic nephropathy by activating TGFbeta1 expression through demethylation of CpG islands in the TGFbeta1 regulatory region PMID: 29705354
    18. early myocardial CTGF mRNA expression (six hours) after Ang-II exposure is likely dependent on latent TGF-beta activation via the canonical Smad-dependent pathway in resident cardiac cells. PMID: 29575960
    19. These results indicate that the hepatocytic expression of TGF-beta and CTGF is mediated by Wnt signalling in Schistosoma japonicum infection. PMID: 28331224
    20. Infection with Mycobacterium bovis results in increase in interleukin-1alpha, TGF-beta1, and MMP1 in multinucleated macrophages. PMID: 29504104
    21. type I and II IFN as in vivo modifiers of LIC fate that may significantly affect the impact of putative leukemia-driving activities such as the ETV6-RUNX1-mediated growth advantage in the presence of TGF-beta and TLR4-upregulated mutagenic activity PMID: 27220664
    22. Studies indicate key developmental processes that require transforming growth factor beta (TGF-beta) signaling [Review]. PMID: 29190317
    23. Osthole,a natural coumarin, decreases the collagen/III ratio in transforming growth factor beta 1 (TGF-beta1)-transfected mouse cardiac fibroblasts via the TGF-beta/Smad signaling pathway. PMID: 29860992
    24. both ERK and Smad2 signal pathways are involved in the activation of macrophages induced by TGF-b1 and high-ambient glucose, while there is no crosstalk shown in the process. PMID: 29199516
    25. In obese mice, periodontitis caused the downregulation of MMP2, and upregulation of TIMP1 and TGF-beta1 at transcriptional and translational levels PMID: 29322806
    26. The protective effect of the EP2 receptor on TGF-beta1 induced podocyte injury via the PI3K / Akt signaling pathway. PMID: 29746568
    27. This study demonstrates that prevention of renal apoB accumulation is a mechanism by which TGF-beta inhibition is nephroprotective. PMID: 28912302
    28. data show that increased TGFbeta in the tumour microenvironment represents a primary mechanism of immune evasion that promotes T-cell exclusion and blocks acquisition of the TH1-effector phenotype; immunotherapies directed against TGFbeta signalling may therefore have broad applications in treating patients with advanced colorectal cancer PMID: 29443964
    29. IL 6 and TGF beta perform essential role in cerebral malaria pathogenesis by modulating the level of glial cell induced neuroinflammation. PMID: 28803696
    30. The increased susceptibility to IMQ-induced psoriasis of GILZ-Tg mice was significantly associated with skin-specific over-activation of TGF-beta1-mediated signaling via SMAD2/3. PMID: 27934944
    31. The data suggest that B cells can down-regulate the function of antigen-presenting cells, and in turn encephalitogenic Th1/Th17 responses, via TGF-beta1. PMID: 27708418
    32. p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF exp p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF expression during tooth development. PMID: 28825193
    33. inhibiting NCAM1 would be cardioprotective, counteract the pathological action of TGFbeta1 and reduce heart failure severity. PMID: 28870505
    34. TGF-beta signaling has a role in inhibiting tumor progression and invasion in an induced mouse bladder cancer model PMID: 27378170
    35. calpains inhibition plays crucial roles in vascular restenosis by preventing neointimal hyperplasia at the early stage via suppression of the MMP2/TGF-beta1 pathway. PMID: 27453531
    36. Suggest an essential role for platelet-derived TGFbeta1 for the vascular remodelling response to arterial injury, apparently independent from the role of platelets in thrombosis or haemostasis. PMID: 28726976
    37. In cultured B16 melanoma and Bend3 endothelial cells treated with Bend3 conditioned media, MITF, tyrosinase, and melanin expression decreased due to TGFB1 secreted by the endothelial cells. PMID: 27172887
    38. Lycat regulates TGF-beta mediated lung fibroblast differentiation in pulmonary fibrosis. PMID: 28751023
    39. TFGbeta1 and arachidonic acid synthase expression is increased during the involution period in mammary glands. PMID: 28381667
    40. Chronic exercise suppressed the TGF-beta1/IkB-alpha axis in the hypothalamus and improved the energy homeostasis in an animal model of obesity-associated with aging. PMID: 28854149
    41. miR-146 inhibits skeletal muscle fibrosis by suppressing TGF-beta/Smad4 signaling pathway. PMID: 28510617
    42. Study shows that expression of Gadd45 family members is adjusted in response to KCl/depolarization, BDNF/TRKB, and/or TGFB-signaling. Additionally, results indicate decreased Gadd45 expression in a mouse model of depression, and that Gadd45b expression and DNA demethylation activate Arc transcription. PMID: 28444170
    43. Bioinformatics analysis provides positive supportive correlation between somatic mutations for VD-related genes and the TGF-beta pathway PMID: 27456065
    44. Taken together, these results indicate that eIF6 may be involved in external mechanical force-mediated murine dermal fibroblast function at least partly through the TGF-beta1/TGFBR1/TGFBR2 pathway. PMID: 27824055
    45. important roles in virtually every stage of neural development [review] PMID: 28130363
    46. These data suggest that the interplay between cell-matrix adhesion and intercellular adhesion is an important determinant for some aspects of TGFbeta1-induced epithelial-mesenchymal transition via alphaSMA expression induction. PMID: 27194451
    47. Excessive activation of TGFbeta by spinal instability causes vertebral endplate sclerosis and intervertebral disk degeneration. PMID: 27256073
    48. A Smad3-PTEN regulatory loop controls proliferation and apoptotic responses to TGF-beta in mouse endometrium. PMID: 28524854
    49. TGFss through the Alk1/TgfssR2 receptor axis is acting on endothelial cells to produce hematopoiesis. PMID: 29253505
    50. The adoptive transfer of NK1.1(-) CD4(+) NKG2D(+) cells suppressed DSS-induced colitis largely dependent on TGF-beta. Thus, NK1.1(-) CD4(+) NKG2D(+) cells exhibited immune regulatory functions, and this T cell subset could be developed to suppress inflammation in clinics. PMID: 28224733

    显示更多

    收起更多

  • 亚细胞定位:
    Secreted, extracellular space, extracellular matrix
  • 蛋白家族:
    TGF-beta family
  • 数据库链接: