Mouse Cystatin-F(CST7) ELISA kit

Datasheet
  • 中文名称:
    小鼠胱抑素F(CST7)ELISA kit
  • 货号:
    CSB-EL006095MO
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 别名:
    Cst7Cystatin-F ELISA Kit; Cystatin-7 ELISA Kit; Cystatin-like metastasis-associated protein ELISA Kit; CMAP ELISA Kit; Leukocystatin ELISA Kit
  • 缩写:
    CST7
  • Uniprot No.:
  • 种属:
    Mus musculus (Mouse)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.156 ng/mL-10 ng/mL
  • 灵敏度:
    0.039 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cell Biology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse CST7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 89
    Range % 84-93
    1:2 Average % 100
    Range % 95-105
    1:4 Average % 87
    Range % 83-90
    1:8 Average % 104
    Range % 100-107
  • 回收率:
    The recovery of mouse CST7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 86 83-89
    EDTA plasma (n=4) 95 91-99
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.547 2.601 2.574 2.452
    5 2.120 2.032 2.076 1.954
    2.5 1.394 1.402 1.398 1.276
    1.25 0.820 0.805 0.813 0.691
    0.625 0.517 0.531 0.524 0.402
    0.312 0.325 0.326 0.326 0.204
    0.156 0.239 0.242 0.241 0.119
    0 0.121 0.123 0.122  
  • 货期:
    7-14 working days

靶点详情

  • 功能:
    Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
  • 基因功能参考文献:
    1. High CST7 expression is associated with Alzheimer's disease. PMID: 28904096
    2. Our results validate cystatin F as a useful biomarker of early pathogenesis in experimental models of prion disease, and point to unexpected species-specific differences in the transcriptional responses to prion infections. PMID: 28178353
    3. During the active remyelinating phase, both CysF knockdown (CysFKD) and microglial-selective CatC overexpression (CatCOE) showed a worsening of the demyelination in Plp(4e/-) transgenic mice. Conversely, during the chronic demyelinating phase, CatC knockdown (CatCKD) ameliorated the demyelination. Our results suggest that the balance between CatC and CysF expression controls the demyelination and remyelination process. PMID: 28251676
    4. The cystatin F expression in the activated microglia is closely associated with the effect of the A2A receptors, which may be related to the neuroinflammatory responses occurring during the pathological process. PMID: 23285090
    5. an inhibitory activity other than cystatin F quenches CatL activity in activated macrophages PMID: 21956111
    6. Expression of cystatin F indicates ongoing demyelination and remyelination; absence of cystatin F expression indicates cessation of remyelination in the demyelinating area. PMID: 21344476

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  • 亚细胞定位:
    Secreted
  • 蛋白家族:
    Cystatin family
  • 数据库链接: