Human Prolactin Inducible Protein(PIP)ELISA Kit

  • 中文名称:
    人催乳素诱导蛋白(PIP)ELISA Kit
  • 货号:
  • 规格:
  • 价格:
  • 其他:


  • 别名:
    BRST-2 ELISA Kit; BRST2 ELISA Kit; GCDFP-15 ELISA Kit; GCDGP15 ELISA Kit; gp17 ELISA Kit; GPIP4 ELISA Kit; Gross cystic disease fluid protein 15 ELISA Kit; PIP ELISA Kit; PIP_HUMAN ELISA Kit; Prolactin induced protein ELISA Kit; Prolactin inducible protein precursor ELISA Kit; Prolactin-induced protein ELISA Kit; Prolactin-inducible protein ELISA Kit; SABP ELISA Kit; Secretory actin binding protein ELISA Kit; Secretory actin-binding protein ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.8 ng/mL-40 ng/mL
  • 灵敏度:
    1 ng/mL
  • 反应时间:
  • 样本体积:
  • 检测波长:
    450 nm
  • 研究领域:
  • 测定原理:
  • 测定方法:
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human PIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 89  
    Range % 84-95  
    1:2 Average % 94  
    Range % 91-100  
    1:4 Average % 97  
    Range % 92-105  
    1:8 Average % 93  
    Range % 84-98  
  • 回收率:
    The recovery of human PIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-100  
    EDTA plasma (n=4) 97 90-103  
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    40 2.030 1.998 2.014 1.965  
    16 1.426 1.476 1.451 1.402  
    6 0.764 0.804 0.784 0.735  
    2 0.391 0.399 0.395 0.346  
    0.8 0.186 0.194 0.190 0.141  
    0 0.049 0.048 0.049    
  • 货期:
    3-5 working days


  • 基因功能参考文献:
    1. Unusual N-type glycosylation of salivary prolactin-inducible protein (PIP): multiple Lewis(Y) epitopes generate highly-fucosylated glycan structures. PMID: 29858715
    2. that GATA3 is a very sensitive marker for primary genital extramammary Paget diseases and is more sensitive than gross cystic disease fluid protein 15 PMID: 28693610
    3. monoclonal antibodies to GATA-3 are very sensitive reagents for the identification of breast CA, surpassing antibodies to GCDFP-15 and mammaglobin A, and offer a significant improvement in identifying TNBCs PMID: 26447897
    4. good coincidence was observed between primary and metastatic tumor GATA3 expression (kappa value = 0.826 >0.75) as compared with the coincidence of GCDFP15 (kappa value =0.492 <0.75) and mammaglobin (kappa value =0.593 <0.75) PMID: 28038704
    5. High expression of GCDFP-15 is associated with molecular apocrine breast cancer. PMID: 26711786
    6. While highly expressed under pathological conditions of the mammary gland, including breast cancers, PIP is expressed in very few other cancers. Although the function of PIP is not well elucidated, numerous studies suggest that its primary role may be related to host defense and immune modulation. Studies in Pip null mice suggest that PIP may play a similar immune-regulatory role in breast cancer. [Review] PMID: 27602994
    7. novel marker GATA3 stains a significantly higher proportion of both primary and metastatic breast carcinomas than GCDFP15 or mammaglobin with stronger and more diffuse staining, helpful in cases with small tissue samples PMID: 25906123
    8. In summary, this study suggests that PIP expression regulates the process of cell adhesion in breast cancer. PMID: 26585492
    9. GCDFP15 in stratum corneum is a potential marker for dysregulated sweating in atopic dermatitis. PMID: 25919462
    10. The commonly used breast carcinoma biomarkers vary in their prognostic implications. GCDFP-15 independently indicated a favourable prognosis. GATA-3 and MGB were not associated with outcome. PMID: 25425335
    11. Data indicate the potential role of Gross cystic disease fluid protein-15/prolactin-inducible protein (GCDFP-15/PIP) as a biomarker for keratoconus (KC)disease. PMID: 25405607
    12. Prolactin-induced protein mediates cell invasion and regulates integrin signaling in estrogen receptor-negative breast cancer. PMID: 22817771
    13. GCDFP-15 expression is higher in tumors with favorable prognostic features. GCDFP-15 expression is further a frequent feature of androgen receptor positive tumors and the molecular apocrine subtype. PMID: 25070172
    14. PIP is a breast cancer-related protein that is expressed in a majority of breast tumors and has a significant function in the biology of this disease. [review] PMID: 25472539
    15. we found that HER2 or GCDFP15 protein overexpression is a sensitive and specific tool to differentiate MA from BL in the context of ER negative tumors. PMID: 23663520
    16. The roles of PIP in the related T47D breast cancer cell line, were investigated. PMID: 23755096
    17. PIP is required for cell cycle progression in breast cancer and provides a rationale for exploring PIP inhibition as a therapeutic approach in breast cancer that can potentially target microtubule polymerization. PMID: 24862759
    18. Report GCDFP15 expression in metastatic basal-like triple negative breast carcinomas. PMID: 24272937
    19. AZGP1 and PIP were found in significantly lower levels in invasive breast tissue. PMID: 24114735
    20. GCDFP-15 was associated significantly with a breast cancer profile of good prognosis tumors. PMID: 23332923
    21. Gross cystic disease fluid protein-15 and mammaglobin A expression determined by immunohistochemistry is of limited utility in triple-negative breast cancer. PMID: 22963676
    22. Interaction analysis identifies semenogelin I fragments as new binding partners of PIP in human seminal plasma PMID: 23085372
    23. GCDFP-15 alone is not a useful marker to detect the metastasis of basaloid type breast cancers. PMID: 23011826
    24. expression was down-regulated in azoospermia, indicating that PIP may be a plausible marker of azoospermia PMID: 22724438
    25. The first report of native human serum albumin (HSA)-PIP complex formation in seminal plasma. PMID: 22209935
    26. abnormal development of the prepuce in hypospadias is associated with variation in PIP expression PMID: 21883842
    27. Data suggest that Runx2 controls a positive feedback loop between androgen signaling and PIP, and pharmacological inhibition of PIP may be useful to treat PIP positive tumors. PMID: 21809344
    28. Most basal-like breast carcinomas and unclassified triple-negative carcinomas are negative for mammaglobin and gross cystic disease fluid protein 15. PMID: 21411781
    29. expressed on a small subset of primary lung adenocarcinomas PMID: 19620839
    30. Intragenic amplification and formation of extrachromosomal small circular DNA molecules from the PIP gene on chromosome 7 in primary breast carcinomas PMID: 11992405
    31. different conformations and/or tissue-specific posttranslational modifications of the proteins, although their primary structure was identical by MS/MS analysis PMID: 12755619
    32. Forms a protein complex with PIG-F and is involved in the conversion of phosphatidylinositol glycans. PMID: 15632136
    33. The sensitivity of mammaglobin is equal or superior to that of GCDFP-15 for investigation of breast carcinoma. PMID: 18251583
    34. analysis of the novel complex formed between zinc alpha2-glycoprotein (ZAG) and prolactin-inducible protein (PIP) from human seminal plasma PMID: 18930737
    35. Both MGB1 and GCDFP-15 are specific markers for metastatic breast carcinomas in cell block fluid specimens (88 vs. 96%). PMID: 19217055
    36. importance of a regulatory subnetwork associated with PIP expression in which STAT5 appears as a potential transcriptional regulator PMID: 19262752
    37. PIP(alternate name GCDFP-15, gp17) is a secretory marker of apocrine differentiation in breast carcinoma. It is an aspartyl proteinase with specificity restricted for fibronectin. PMID: 10713110



  • 亚细胞定位:
  • 蛋白家族:
    PIP family
  • 组织特异性:
    Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
  • 数据库链接:

    HGNC: 8993

    OMIM: 176720

    KEGG: hsa:5304

    STRING: 9606.ENSP00000291009

    UniGene: Hs.99949