Human SARS-CoV-2 N IgG Antibody ELISA Kit

Datasheet
  • 中文名称:
    人新型冠状病毒核蛋白(SARS-CoV-2 N) IgG抗体 ELISA Kit
  • 货号:
    CSB-EL3325HU
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 产品描述:

    CUSABIO’s Human SARS-CoV-2 nucleoprotein (N) IgG Antibody ELISA Kit allows for in vitro qualitative detection of the IgG antibody-specific for SARS-CoV-2 N protein in human serum and plasma. This research-use only kit can be applied in the studies associated with COVID-19, infectious and severe pneumonia caused by SARS-CoV-2. It has been validated with high sensitivity, high specificity, and high precision.

    This assay mechanism is based on the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with human SARS-CoV-2 nucleoprotein. Samples are pipetted into the wells with anti-human IgG conjugated HRP. Following a thorough wash, the TMB Substrate solution is added to the wells, eliciting a color change. The color develops in proportion to the amount of human SARS-CoV-2 N IgG antibody bound in the initial step. The addition of the stop solution to the wells terminates the color reaction. The color intensity can be measured at 450 nm via a microplate reader. The SARS-CoV-2 N IgG antibody titer in the samples is determined by referring to the negative control. It indicates the presence of SARS-CoV-2 N IgG antibody if the sample O.D. (optical density) is greater than or equal to the cutoff value (2.1×Average O.D. value of negative control). There is no SARS-CoV-2 N IgG antibody present in the sample if the O.D. is less than the cutoff value.

    Nucleoprotein is the most abundant protein in SARS-CoV-2. It packs the positive-sense single-stranded viral RNA into a helical ribonucleocapsid (RNP), forming the viral nucleocapsid (N). The N protein plays an important role in improving the efficiency of virus transcription and assembly. It is also involved in viral pathogenesis. N protein possessing an immunogenic effect makes it trigger an immune response, which leads to the production of IgM/G against SARA-CoV-2 N protein. SARS-CoV-2 N IgG antibodies appear in the serum after 2-3 weeks after the onset of the symptoms, peaks within the third week, and gradually declines to a lower level. IgG-specific for SARS-CoV-2 N protein rapidly increases to a higher level after a secondary infection and retains in the body for several months even years.

  • 别名:
    2019-nCoV; SARS-CoV-2; COVID-19; 2019 Novel Coronavirus; Coronavirus
  • 缩写:
    SARS-CoV-2 N Ab (IgG)
  • Uniprot No.:
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Infectious Diseases
  • 测定原理:
    qualitative
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<15%

    Three samples of known concentration were tested in twenty assays to assess.

  • 标准曲线:

    Test parameter

    specification

    test result

    Positive control

    0.6

    0.867

    Negative control

    0.25

    0.184

    Positive rate

    20,Positive

    100%

    Negative rate

    20,Negative

    100%

  • 本试剂盒所含材料:
    • A 96-well Coated assay plate --This microplate has been pre-coated with human SARS-CoV-2 nucleoprotein.
    • Negative Control (1 x 800 μl) --It is free of the SARS-CoV-2 N IgG antibody and used to preclude the false positive.
    • Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of the test results.
    • HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
    • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated anti-Human IgG antibody.
    • Sample Diluent (2 x 20 ml) --Dilute the sample solution.
    • Wash Buffer (25 x concentrate) (1 x 20 ml) --Wash the unbound reagents.
    • TMB Substrate (1 x 10 ml) --React with HRP, eliciting a chromogenic color reaction.
    • Stop Solution (1 x 10 ml) --Stop the color reaction. The solution turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells) --Seal the microplate when incubation.
    • An Instruction manual

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  • 本试剂盒不含材料:
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer.
    • Absorbent paper for blotting the microtiter plate.
    • 50-300ul multi-channel micropipette
    • 100ml and 500ml graduated cylinders.
    • Deionized or distilled water.
    • Pipette tips
    • Timer
    • Test tubes for dilution.

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  • 货期:
    3-5 working days