Human SARS-CoV-2 N IgG Antibody ELISA Kit

^Datasheet

中文名称：

人新型冠状病毒核蛋白(SARS-CoV-2 N) IgG抗体 ELISA Kit
货号：

CSB-EL3325HU
规格：

96T
价格：

3600
其他：

产品详情
相关产品

产品详情

产品描述：

CUSABIO’s Human SARS-CoV-2 nucleoprotein (N) IgG Antibody ELISA Kit allows for in vitro qualitative detection of the IgG antibody-specific for SARS-CoV-2 N protein in human serum and plasma. This research-use only kit can be applied in the studies associated with COVID-19, infectious and severe pneumonia caused by SARS-CoV-2. It has been validated with high sensitivity, high specificity, and high precision.

This assay mechanism is based on the qualitative enzyme immunoassay technique. The microtiter plate has been pre-coated with human SARS-CoV-2 nucleoprotein. Samples are pipetted into the wells with anti-human IgG conjugated HRP. Following a thorough wash, the TMB Substrate solution is added to the wells, eliciting a color change. The color develops in proportion to the amount of human SARS-CoV-2 N IgG antibody bound in the initial step. The addition of the stop solution to the wells terminates the color reaction. The color intensity can be measured at 450 nm via a microplate reader. The SARS-CoV-2 N IgG antibody titer in the samples is determined by referring to the negative control. It indicates the presence of SARS-CoV-2 N IgG antibody if the sample O.D. (optical density) is greater than or equal to the cutoff value (2.1×Average O.D. value of negative control). There is no SARS-CoV-2 N IgG antibody present in the sample if the O.D. is less than the cutoff value.

Nucleoprotein is the most abundant protein in SARS-CoV-2. It packs the positive-sense single-stranded viral RNA into a helical ribonucleocapsid (RNP), forming the viral nucleocapsid (N). The N protein plays an important role in improving the efficiency of virus transcription and assembly. It is also involved in viral pathogenesis. N protein possessing an immunogenic effect makes it trigger an immune response, which leads to the production of IgM/G against SARA-CoV-2 N protein. SARS-CoV-2 N IgG antibodies appear in the serum after 2-3 weeks after the onset of the symptoms, peaks within the third week, and gradually declines to a lower level. IgG-specific for SARS-CoV-2 N protein rapidly increases to a higher level after a secondary infection and retains in the body for several months even years.
别名：

2019-nCoV; SARS-CoV-2; COVID-19; 2019 Novel Coronavirus; Coronavirus
缩写：

SARS-CoV-2 N Ab (IgG)
Uniprot No.：

P0DTC9
种属：

Homo sapiens (Human)
样本类型：

serum, plasma
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Infectious Diseases
测定原理：

qualitative

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

标准曲线：

Test parameter	specification	test result
Positive control	≥0.6	0.867
Negative control	≤0.25	0.184
Positive rate	20,Positive	100%
Negative rate	20,Negative	100%

本试剂盒所含材料：
- A 96-well Coated assay plate --This microplate has been pre-coated with human SARS-CoV-2 nucleoprotein.
- Negative Control (1 x 800 μl) --It is free of the SARS-CoV-2 N IgG antibody and used to preclude the false positive.
- Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of the test results.
- HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
- HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated anti-Human IgG antibody.
- Sample Diluent (2 x 20 ml) --Dilute the sample solution.
- Wash Buffer (25 x concentrate) (1 x 20 ml) --Wash the unbound reagents.
- TMB Substrate (1 x 10 ml) --React with HRP, eliciting a chromogenic color reaction.
- Stop Solution (1 x 10 ml) --Stop the color reaction. The solution turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --Seal the microplate when incubation.
- An Instruction manual
显示更多

收起更多
本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer.
- Absorbent paper for blotting the microtiter plate.
- 50-300ul multi-channel micropipette
- 100ml and 500ml graduated cylinders.
- Deionized or distilled water.
- Pipette tips
- Timer
- Test tubes for dilution.
显示更多

收起更多
货期：

3-5 working days

Human SARS-CoV-2 N IgG Antibody ELISA Kit

产品详情

相关产品

Most popular with customers

产品中心

信号通路

技术信息

联系信息