Human Coenzyme Q10 (CoQ10) ELISA Kit

Datasheet
  • 中文名称:
    人辅酶Q10 (CoQ10) ELISA Kit
  • 货号:
    CSB-E14081h
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 别名:
    N/A
  • 缩写:
    CoQ10
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    3.12 ng/mL-50 ng/mL
  • 灵敏度:
    1.56 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:20 Average % 96  
    Range % 91-101  
    1:40 Average % 99  
    Range % 93-104  
    1:80 Average % 92  
    Range % 87-96  
    1:160 Average % 90  
    Range % 85-94  
  • 回收率:
    The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 84 80-88  
    EDTA plasma (n=4) 93 88-97  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.112 0.103 0.108    
    25 0.208 0.224 0.216    
    12.5 0.377 0.397 0.387    
    6.25 0.783 0.718 0.751    
    3.12 1.121 1.086 1.104    
    0 1.951 1.829 1.890    
  • 货期:
    3-5 working days

问答及客户评论

 常见问题解答
Q:

Please provide the following information for Human Coenzyme Q10, CoQ10 ELISA Kit (cat:CSB-E14081h):
1.What is the standard?
2.What is the antibody in this kit?
3. Is the antibody against lipid Coq10 or to its binding protein?
4. will binding protein be labeled to Co1q10 or directly coq10?

A:
Thanks for your inquiry.
The standard is purified CoQ10.
This kit detects total CoQ10, and the antibody in the kit is mouse monoclonal antibody.
The antibody is specific to CoQ10.Pls let me know if you have any further questions. Thank you.
 客户评论
  • Sample type: Serum
    Sample species: Human
    Review: There was no constistency between dilutions of the same sample. The variability was large. Plus, the values of the healthy controls was outside of normal values (1:10 dilution gave 0.25 ug/ml, while 1:100 gave 2.56 ug/ml). Also, because of the negative correlation between concentration and absorbance, very difficult to calculate concentration of the sample. The only good thing is that the time required to measure coQ10 is short.