Human 8-iso prostaglandin F2α,8-iso-PGF2a ELISA Kit

Datasheet
  • 中文名称:
    人8异前列腺素F2α(8-iso-PGF2a)ELISA kit
  • 货号:
    CSB-E12100h
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 别名:
    8-iso prostaglandin F2α,8-iso prostaglandin F2 alpha,8-iso-PGF2α,8-iso-PGF2 alpha
  • 缩写:
    8-iso-PGF2a
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    125 pg/mL-5000 pg/mL
  • 灵敏度:
    125 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human 8-iso-PGF2a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 90-100  
    1:2 Average % 92  
    Range % 85-99  
    1:4 Average % 95  
    Range % 90-103  
    1:8 Average % 94  
    Range % 90-99  
  • 回收率:
    The recovery of human 8-iso-PGF2a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 88-102  
    EDTA plasma (n=4) 97 91-103  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    5000 0.124 0.126 0.125    
    2500 0.219 0.232 0.226    
    1150 0.341 0.354 0.348    
    375 0.605 0.646 0.626    
    125 1.273 1.305 1.289    
    0 2.654 2.551 2.603    
  • 货期:
    3-5 working days