Human 2,3-Diphosphoglycerate,2,3-DPG ELISA Kit

Datasheet
  • 中文名称:
    人2,3-二磷酸甘油酸(2,3-DPG)ELISA Kit
  • 货号:
    CSB-E09861h
  • 规格:
    96T
  • 价格:
    3600
  • 其他:

产品详情

  • 缩写:
    2,3DPG
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围:
    0.312 μmol/mL-20 μmol/mL
  • 灵敏度:
    0.078 μmol/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human 2, 3-DPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 96  
    Range % 92-100  
    1:2 Average % 98  
    Range % 92-103  
    1:4 Average % 95  
    Range % 90-99  
    1:8 Average % 90  
    Range % 85-96  
  • 回收率:
    The recovery of human 2, 3-DPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 89 85-94  
    EDTA plasma (n=4) 84 80-89  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    \"\"
    µmol/ml OD1 OD2 Average Corrected  
    20 2.873 2.712 2.793 2.636  
    10 2.265 2.149 2.207 2.050  
    5 1.372 1.328 1.350 1.193  
    2.5 0.854 0.813 0.834 0.677  
    1.25 0.457 0.481 0.469 0.312  
    0.625 0.337 0.357 0.347 0.190  
    0.312 0.202 0.207 0.205 0.048  
    0 0.161 0.152 0.157    
  • 货期:
    3-5 working days

问答及客户评论

 常见问题解答
Q:

Could you please advise me the below:
“you had any data to support using this kit for samples from Red Blood Cell units? These blood units typically are collected/stored with a citrate anticoagulant. We currently use a deproteinzation procedure to extract the DPG from the cells.”
Any/all feedback you might have would be greatly appreciated!

A:
Thanks for your inquiry.
We validated normal human serum before, but sorry we didn't validate Red Blood Cell(RBC)before. Some customers inquired about this before, but they didn't let us know the validation information and test values because they could not disclose it.
If you want to test RBC,
①Generally deproteinzation procedure is not needed. The specificity of raw materials used in the kit has been validated before. It can only recognize 2,3-DPG;
②If deproteinzation procedure has been conducted, then you can continue the asasy. It's better to do a pretest first.
Pls let me know if you have any further questions. Thank you.