Fish 17-hydroxypregnenolone ELISA kit

Datasheet
  • 中文名称:
    鱼17-羟孕烯醇酮ELISA kit
  • 货号:
    CSB-EQ027292FI
  • 规格:
    96T
  • 价格:
    4200
  • 其他:

产品详情

  • 别名:
    17-OH-pregnenolone, 17α-hydroxypregnenolone
  • 缩写:
    17-hydroxypregnenolone
  • 种属:
    Fish
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.9 ng/mL-50 ng/mL
  • 灵敏度:
    1.9 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Others
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of fish 17-hydroxypregnenolone in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 84-107  
    1:2 Average % 93  
    Range % 85-103  
    1:4 Average % 95  
    Range % 88-100  
    1:8 Average % 92  
    Range % 82-101  
  • 回收率:
    The recovery of fish 17-hydroxypregnenolone spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 86-108  
    EDTA plasma (n=4) 93 89-99  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.136 0.143 0.140    
    23.1 0.237 0.248 0.243    
    11.5 0.353 0.364 0.359    
    6.2 0.605 0.591 0.598    
    1.9 1.283 1.228 1.256    
    0 2.682 2.702 2.692    
  • 货期:
    7-14 working days