Dog Interleukin 6,IL-6 ELISA Kit

Datasheet
  • 中文名称:
    犬白介素6(IL-6)ELISA Kit
  • 货号:
    CSB-E11260c
  • 规格:
    96T
  • 价格:
    3800
  • 其他:

产品详情

  • 别名:
    IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
  • 缩写:
    IL6
  • Uniprot No.:
  • 种属:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    1.56 pg/mL-100 pg/mL
  • 灵敏度:
    0.39 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %84
    Range %80-88
    1:2Average %95
    Range %89-105
    1:4Average %100
    Range %95-106
    1:8Average %93
    Range %89-97
  • 回收率:
    The recovery of dog IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8782-96
    EDTA plasma (n=4)9385-98
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.954 2.989 2.972 2.855
    502.388 2.386 2.387 2.270
    251.758 1.796 1.777 1.660
    12.51.035 1.106 1.071 0.954
    6.250.502 0.513 0.508 0.391
    3.120.271 0.268 0.270 0.153
    1.560.184 0.196 0.190 0.073
    00.112 0.121 0.117  
  • 货期:
    3-5 working days

靶点详情

  • 功能:
    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
  • 基因功能参考文献:
    1. An increase in ventricular dimension is accompanied by increases in AQP4 and IL-6 concentrations in the CSF of dogs with idiopathic communicating hydrocephalus. All were greatly reduced or normalised after shunt treatment. Therefore AQP4 and IL-6 in CSF can be indicators of ventriculomegaly, of cellular damage, and of improvement after the treatment of hydrocephalus. PMID: 27357498
    2. The results of this study suggest that IL-6 is a useful prognostic marker for canine critical care patients with acute internal disease. PMID: 25866911
    3. This diminished TNF-alpha, IL-6 and iNOS expression, and NO production was in parallel to the respective decreased NF-kappaB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 microM and 50 microM propofol. PMID: 25312048
    4. Release of interleukin-6 and substance P may initiate proinflammatory effects leading to development of persistent pain in dogs with syringomyelia. PMID: 23659719
    5. The etiology of blindness in dogs with pituitary dependent hypercortisolism, including the role of adiponectin, interleukin 6, insulin, and nitric oxide, is reported. PMID: 22381588
    6. There was no association between IL6 variants and periodontal disease in dogs. PMID: 21708229
    7. These findings suggest that IL-6 secretion during acute urinary tract infection may serve a dual biological role by initiating the inflammatory response while also repairing urothelial defenses. PMID: 21115724
    8. Tumor-infiltrating lymphocytes isolated from regressing canine transmissible venereal tumor secrete high concentrations of IL-6 and antagonize the anti-lymphokine-activated killer activity of tumor cell transforming growth factor-beta 1. PMID: 14734728
    9. Plasma IL-6 concentration is predictive of outcome in canine systemic inflammatory response syndrome and sepsis and may be a valuable laboratory parameter for assessing critically ill dogs. PMID: 17806073
    10. Adipose tissue-derived mesenchymal stem cells expressed the mRNA of transforming growth factor beta (TGF-beta), IL-6, IL-8, CCL2, CCL5, VEGF,HGF, tissue inhibitor metalloproteinase-1/2, and cyclooxygenase-2 but not that of IL-10. PMID: 18717642

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  • 亚细胞定位:
    Secreted
  • 蛋白家族:
    IL-6 superfamily
  • 数据库链接: